Human EDIL3 ELISA Kit | EDIL3 elisa kit

Human EDIL3 ELISA Kit

Cat. Num. AAA8248349

• Gene Names: EDIL3; DEL1
• Reactivity: Human
• Synonyms: EDIL3; Human EDIL3 ELISA Kit; DEL1; EGF-like repeat and discoidin I-like domain-containing protein 3; Developmentally-regulated endothelial cell locus 1 protein; Integrin-binding protein DEL1; EDIL3 elisa kit

• Reactivity: Human
• Specificity: The Human EDIL3 ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human EDIL3 proteins within the range of 15.6 pg/ml - 1000 pg/ml.
• Sequence Length: 470

• Samples: Cell Culture Supernates, Serum, Plasma
• Sensitivity: <10 pg/ml
• Preparation and Storage: Shipped and store at 4 degree C for 6 months, store at -20 degree C for one year. Avoid freeze/thaw cycles.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for EDIL3 elisa kit

Principle of the Assay: The Human EDIL3 ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human EDIL3 in Cell Culture Supernates, Serum, Plasma. This assay employs an antibody specific for Human EDIL3 coated on a 96-well plate. Standards and samples are pipetted into the wells and EDIL3 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human EDIL3 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of EDIL3 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

Background: EDIL3, also known as DEL1, is a glycoprotein that is secreted by endothelial cells and can associate with the endothelial cell surface and the extracellular matrix. It is regulated by hypoxia or vascular injury and has been implicated in vascular remodeling during angiogenesis. The EDIL3 gene is mapped to 5q14. Human endothelial cells bind to Del1 together with the alpha-V (ITGAV)/beta-3 (ITGB3) integrin receptor. It is confirmed that adhesion of endothelial cells to Del1 is integrin mediated and that Del1 binds ITGAV/ITGB3. Functional analysis showed that both Del1 and vitronectin (VTN) stimulate endothelial cell migration. Immunoprecipitation and immunoblot analyses revealed that adhesion of endothelial cells to Del1 induces tyrosine phosphorylation of PTK2.

NCBI and Uniprot Product Information

• NCBI GI #: 522023554
• NCBI GeneID: 10085
• NCBI Accession #: NP_001265571.1
• NCBI GenBank Nucleotide #: NM_001278642.1
• UniProt Accession #: O43854; O43855; Q5D094; Q8N610; B2R763
• Molecular Weight: 52,748 Da
• NCBI Official Full Name: EGF-like repeat and discoidin I-like domain-containing protein 3 isoform 2
• NCBI Official Synonym Full Names: EGF like repeats and discoidin domains 3
• NCBI Official Symbol: EDIL3
• NCBI Official Synonym Symbols: DEL1
• NCBI Protein Information: EGF-like repeat and discoidin I-like domain-containing protein 3
• UniProt Protein Name: EGF-like repeat and discoidin I-like domain-containing protein 3
• Protein Family: EGF-like repeat and discoidin I-like domain-containing protein
• UniProt Gene Name: EDIL3
• UniProt Synonym Gene Names: DEL1
• UniProt Entry Name: EDIL3_HUMAN

NCBI Description

The protein encoded by this gene is an integrin ligand. It plays an important role in mediating angiogenesis and may be important in vessel wall remodeling and development. It also influences endothelial cell behavior. [provided by RefSeq, Jul 2008]

Uniprot Description

EDIL3: Promotes adhesion of endothelial cells through interaction with the alpha-v/beta-3 integrin receptor. Inhibits formation of vascular-like structures. May be involved in regulation of vascular morphogenesis of remodeling in embryonic development. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 5q14

Cellular Component: extracellular matrix