Human E2F Transcription Factor 1 (E2F1) ELISA Kit | E2F1 elisa kit

Human E2F Transcription Factor 1 (E2F1) ELISA Kit

Cat. Num. AAA2023662

• Gene Names: E2F1; RBP3; E2F-1; RBAP1; RBBP3
• Reactivity: Human
• Synonyms: E2F Transcription Factor 1 (E2F1); Human E2F Transcription Factor 1 (E2F1) ELISA Kit; RBBP3; RBP3; RBAP-1; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; pRB-binding protein E2F-1; E2F1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of E2F Transcription Factor 1 (E2F1).; No significant cross-reactivity or interference between E2F Transcription Factor 1 (E2F1) and analogues was observed.
• Sequence Length: 437

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.156-10g/mL
• Sensitivity: < 0.054g/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level E2F Transcription Factor 1 (E2F1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level E2F Transcription Factor 1 (E2F1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for E2F1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to E2F Transcription Factor 1 (E2F1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to E2F Transcription Factor 1 (E2F1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain E2F Transcription Factor 1 (E2F1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E2F Transcription Factor 1 (E2F1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for E2F1 elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 37589126
• NCBI GeneID: 1869
• UniProt Accession #: Q01094; Q13143; Q92768
• Molecular Weight: 46,920 Da
• NCBI Official Full Name: E2F transcription factor 1
• NCBI Official Synonym Full Names: E2F transcription factor 1
• NCBI Official Symbol: E2F1
• NCBI Official Synonym Symbols: RBP3; E2F-1; RBAP1; RBBP3
• NCBI Protein Information: transcription factor E2F1
• UniProt Protein Name: Transcription factor E2F1
• Protein Family: Transcription factor
• UniProt Gene Name: E2F1
• UniProt Synonym Gene Names: RBBP3; E2F-1; RBAP-1; RBBP-3
• UniProt Entry Name: E2F1_HUMAN

NCBI Description

The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. The E2F proteins contain several evolutionally conserved domains found in most members of the family. These domains include a DNA binding domain, a dimerization domain which determines interaction with the differentiation regulated transcription factor proteins (DP), a transactivation domain enriched in acidic amino acids, and a tumor suppressor protein association domain which is embedded within the transactivation domain. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis. [provided by RefSeq, Jul 2008]

Uniprot Description

E2F1: a member of the E2F/DP family of transcription factors.The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor proteins and is also a target of the transforming proteins of small DNA tumor viruses. Binds DNA cooperatively with Dp transcription factors in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication.The Dp-1/E2F complex functions in the control of cell-cycle progression from G1 to S phase. The E2F-1 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. It can mediate both cell proliferation and p53-dependent apoptosis.

Protein type: DNA-binding; Transcription factor

Chromosomal Location of Human Ortholog: 20q11.2

Cellular Component: nucleoplasm; Rb-E2F complex; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; sequence-specific DNA binding; transcription factor binding; transcription factor activity

Biological Process: Notch signaling pathway; transcription, DNA-dependent; apoptosis; positive regulation of transcription, DNA-dependent; mRNA stabilization; negative regulation of transcription from RNA polymerase II promoter; anoikis; positive regulation of fibroblast proliferation; regulation of transcription, DNA-dependent; DNA damage response, signal transduction resulting in induction of apoptosis; forebrain development; DNA damage checkpoint; positive regulation of transcription from RNA polymerase II promoter; spermatogenesis; mitotic cell cycle; negative regulation of transcription, DNA-dependent; G1/S transition of mitotic cell cycle