Rabbit anti-Human GCLC Polyclonal Antibody | anti-GCLC antibody

GCLC, NT (GCLC, GLCL, GLCLC, Glutamate-cysteine ligase catalytic subunit, GCS heavy chain, Gamma-ECS, Gamma-glutamylcysteine synthetase)

Cat. Num. AAA646746

• Gene Names: GCLC; GCL; GCS; GLCL; GLCLC
• Reactivity: Human
• Applications: ELISA, Western Blot, Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence
• Purity: Affinity Purified; Purified by Protein A affinity chromatography.
• Synonyms: GCLC; Polyclonal Antibody; NT (GCLC; GLCL; GLCLC; Glutamate-cysteine ligase catalytic subunit; GCS heavy chain; Gamma-ECS; Gamma-glutamylcysteine synthetase); Anti -GCLC; anti-GCLC antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Human
• Purity/Purification: Affinity Purified; Purified by Protein A affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide.

• Applicable Applications for anti-GCLC antibody: ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Immunofluorescence (IF)
• Application Notes: Suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, ELISA; Dilution: ELISA: 1:1,000; Western Blot: 1:100-500; Immunohistochemistry: 1:10-50; Immunofluorescence: 1:10-50; Flow Cytometry: 1:10-50
• Immunogen: GCLC antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 6-34 amino acids from the N-terminal region of human GCLC.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(GCLC Antibody (N-term) western blot analysis in Jurkat cell line lysates (35ug/lane).This demonstrates the GCLC antibody detected the GCLC protein (arrow).)

Immunohistochemistry (IHC)

(GCLC Antibody (N-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human esophageal carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of GCLC Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Immunofluorescence (IF)

(Confocal immunofluorescent analysis of GCLC Antibody (N-term) with U-251MG cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

Flow Cytometry (FC/FACS)

(GCLC Antibody (N-term) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-GCLC antibody

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.

Product Categories/Family for anti-GCLC antibody

Antibodies; Abs to Amino Acids

NCBI and Uniprot Product Information

• NCBI GI #: 308199423
• NCBI GeneID: 2729
• NCBI Accession #: NP_001184044.1
• NCBI GenBank Nucleotide #: NM_001197115.1
• UniProt Accession #: P48506; Q14399
• Molecular Weight: 72,766 Da
• NCBI Official Full Name: glutamate--cysteine ligase catalytic subunit isoform b
• NCBI Official Synonym Full Names: glutamate-cysteine ligase, catalytic subunit
• NCBI Official Symbol: GCLC
• NCBI Official Synonym Symbols: GCL; GCS; GLCL; GLCLC
• NCBI Protein Information: glutamate--cysteine ligase catalytic subunit; gamma-ECS; GCS heavy chain; gamma-glutamylcysteine synthetase
• UniProt Protein Name: Glutamate--cysteine ligase catalytic subunit
• Protein Family: Glutamate--cysteine ligase
• UniProt Gene Name: GCLC
• UniProt Synonym Gene Names: GLCL; GLCLC
• UniProt Entry Name: GSH1_HUMAN

NCBI Description

Glutamate-cysteine ligase, also known as gamma-glutamylcysteine synthetase is the first rate-limiting enzyme of glutathione synthesis. The enzyme consists of two subunits, a heavy catalytic subunit and a light regulatory subunit. This locus encodes the catalytic subunit, while the regulatory subunit is derived from a different gene located on chromosome 1p22-p21. Mutations at this locus have been associated with hemolytic anemia due to deficiency of gamma-glutamylcysteine synthetase and susceptibility to myocardial infarction.[provided by RefSeq, Oct 2010]

Uniprot Description

Catalytic activity: ATP + L-glutamate + L-cysteine = ADP + phosphate + gamma-L-glutamyl-L-cysteine.

Enzyme regulation: Feedback inhibition by glutathione.

Pathway: Sulfur metabolism; glutathione biosynthesis; glutathione from L-cysteine and L-glutamate: step 1/2.

Subunit structure: Heterodimer of a catalytic heavy chain and a regulatory light chain.

Involvement in disease: Hemolytic anemia due to gamma-glutamylcysteine synthetase deficiency (HAGGSD) [MIM:230450]: A disease characterized by hemolytic anemia, glutathione deficiency, myopathy, late-onset spinocerebellar degeneration, and peripheral neuropathy.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.6 Ref.10 Ref.11

Sequence similarities: Belongs to the glutamate--cysteine ligase type 3 family.