Human Dual Specificity Protein Kinase CLK1 (CLK1) ELISA Kit | CLK1 elisa kit

Human Dual Specificity Protein Kinase CLK1 (CLK1) ELISA Kit

Cat. Num. AAA9715941

• Gene Names: CLK1; CLK; STY; CLK/STY
• Reactivity: Human
• Synonyms: Dual Specificity Protein Kinase CLK1 (CLK1); Human Dual Specificity Protein Kinase CLK1 (CLK1) ELISA Kit; CLK1; CLK; CLK/STY; STY; CDC28/CDC2-like kinase; dual specificity protein kinase CLK1; protein tyrosine kinase STY; CLK1 elisa kit

• Reactivity: Human
• Specificity: Human Dual specificity protein kinase CLK1 (CLK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human CLK1. No significant cross-reactivity or interference between Human CLK1 and analogues was observed.
• Sequence Length: 526

• Assay Type: Sandwich ELISA (Quantitative)
• Samples: Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
• Detection Method: Colorimetric
• Usage Notes: * Do not mix components from different kit lots or use reagents beyond the kit expiration date.; * Allow all reagents to warm to room temperature for at least 30 minutes before opening.; * Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.; * Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.; * Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.; * It is recommended that all samples and standards be assayed in duplicate or triplicate.
• Preparation and Storage: Store at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

(Fig.1. Human Dual specificity protein kinase CLK1 (CLK1) Standard Curve.)

Related Product Information for CLK1 elisa kit

Description: This Human Dual specificity protein kinase CLK1 (CLK1) ELISA Kit employs a two-site sandwich ELISA to quantitate CLK1 in samples. An antibody specific for CLK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CLK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CLK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CLK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

Background: DCAMKL1 is a microtubule-associated kinase that can undergo autophosphorylation. DCAMKL1 also has microtubule-polymerizing activity that is independent of its protein kinase activity. The KIAA0369 cDNA encodes a 740-amino acid peptide that shares 72.3% amino acid identity with doublecortin. The protein has a doublecortin domain, a serine/proline-rich domain, and a calmodulin-dependent kinase-like domain. The authors also identified 4 splice variants of KIAA0369. All splice variants were expressed predominantly in fetal brain, with transcript sizes ranging from 8.0 to 5.0 kb. Varying expression of all isoforms was also seen in all 15 regions of adult human brain examined, with greatest expression seen in cerebral cortex, occipital pole, frontal lobe, amygdala, and hippocampus.

NCBI and Uniprot Product Information

• NCBI GI #: 241666392
• NCBI GeneID: 1195
• NCBI Accession #: NP_001155879.1
• NCBI GenBank Nucleotide #: NP_001155879.1
• UniProt Accession #: P49759
• NCBI Official Full Name: dual specificity protein kinase CLK1 isoform 2
• NCBI Official Synonym Full Names: CDC like kinase 1
• NCBI Official Symbol: CLK1
• NCBI Official Synonym Symbols: CLK; STY; CLK/STY
• NCBI Protein Information: dual specificity protein kinase CLK1
• UniProt Protein Name: Dual specificity protein kinase CLK1
• Protein Family: Dual specificity protein kinase
• UniProt Gene Name: CLK1
• UniProt Synonym Gene Names: CLK
• UniProt Entry Name: CLK1_HUMAN

NCBI Description

This gene encodes a member of the CDC2-like (or LAMMER) family of dual specificity protein kinases. In the nucleus, the encoded protein phosphorylates serine/arginine-rich proteins involved in pre-mRNA processing, releasing them into the nucleoplasm. The choice of splice sites during pre-mRNA processing may be regulated by the concentration of transacting factors, including serine/arginine rich proteins. Therefore, the encoded protein may play an indirect role in governing splice site selection. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jun 2009]

Uniprot Description

CLK1: a CMGC kinase of the CLK family. Phosphorylates serine- and arginine-rich (SR) proteins of the spliceosomal complex. May play a role in regulating RNA splicing.

Protein type: Protein kinase, CMGC; Protein kinase, dual-specificity (non-receptor); EC 2.7.12.1; Kinase, protein; CMGC group; CLK family

Chromosomal Location of Human Ortholog: 2q33

Cellular Component: cytoplasm; nucleus

Molecular Function: protein serine/threonine kinase activity; protein serine/threonine/tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; ATP binding

Biological Process: cell proliferation; peptidyl-serine phosphorylation; peptidyl-tyrosine phosphorylation; regulation of RNA splicing; protein amino acid autophosphorylation; peptidyl-threonine phosphorylation