Human DNA Polymerase Delta Catalytic Subunit ELISA Kit | POLD1 elisa kit

Human DNA Polymerase Delta Catalytic Subunit ELISA Kit

Cat. Num. AAA3804835

• Gene Names: POLD1; CDC2; MDPL; POLD; CRCS10
• Reactivity: Human
• Synonyms: DNA Polymerase Delta Catalytic Subunit; Human DNA Polymerase Delta Catalytic Subunit ELISA Kit; POLD1 elisa kit

• Reactivity: Human
• Sequence Length: 1107

• Samples: Serum, plasma, cell culture supernate and other biological fluids
• Assay Type: Sandwich
• Detection Range: 125 pg/ml-2000 pg/ml
• Sensitivity: 10 pg/ml

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for POLD1 elisa kit

Intended Use: The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration ofDNA POLYMERASE DELTA CATALYTIC SUBUNITin the sample, This DNA POLYMERASE DELTA CATALYTIC SUBUNITELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versusDNA POLYMERASE DELTA CATALYTIC SUBUNITconcentration. The concentration ofDNA POLYMERASE DELTA CATALYTIC SUBUNITin the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 156616275
• NCBI GeneID: 5424
• NCBI Accession #: NP_002682
• NCBI GenBank Nucleotide #: NP_002682.2
• UniProt Accession #: P28340
• NCBI Official Full Name: DNA polymerase delta catalytic subunit isoform 1
• NCBI Official Synonym Full Names: DNA polymerase delta 1, catalytic subunit
• NCBI Official Symbol: POLD1
• NCBI Official Synonym Symbols: CDC2; MDPL; POLD; CRCS10
• NCBI Protein Information: DNA polymerase delta catalytic subunit
• UniProt Protein Name: DNA polymerase delta catalytic subunit
• Protein Family: DNA polymerase
• UniProt Gene Name: POLD1
• UniProt Synonym Gene Names: POLD
• UniProt Entry Name: DPOD1_HUMAN

NCBI Description

This gene encodes the 125-kDa catalytic subunit of DNA polymerase delta. DNA polymerase delta possesses both polymerase and 3' to 5' exonuclease activity and plays a critical role in DNA replication and repair. Alternatively spliced transcript variants have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 6. [provided by RefSeq, Mar 2012]

Uniprot Description

POLD1: Possesses two enzymatic activities: DNA synthesis (polymerase) and an exonucleolytic activity that degrades single stranded DNA in the 3'- to 5'-direction. Required with its accessory proteins (proliferating cell nuclear antigen (PCNA) and replication factor C (RFC) or activator 1) for leading strand synthesis. Also involved in completing Okazaki fragments initiated by the DNA polymerase alpha/primase complex. Belongs to the DNA polymerase type-B family.

Protein type: EC 2.7.7.7; Transferase; DNA repair, damage; Nucleotide Metabolism - pyrimidine; Hydrolase; Nucleotide Metabolism - purine; DNA replication

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; delta DNA polymerase complex; nucleotide-excision repair complex; membrane; cytoplasm; nucleus

Molecular Function: protein binding; DNA binding; metal ion binding; 4 iron, 4 sulfur cluster binding; nucleotide binding; DNA-directed DNA polymerase activity; 3'-5'-exodeoxyribonuclease activity; chromatin binding

Biological Process: mismatch repair; DNA strand elongation during DNA replication; DNA repair; base-excision repair, gap-filling; telomere maintenance via semi-conservative replication; DNA synthesis during DNA repair; DNA replication proofreading; nucleotide-excision repair; base-excision repair; fatty acid homeostasis; transcription-coupled nucleotide-excision repair; telomere maintenance via recombination; mitotic cell cycle; nucleotide-excision repair, DNA gap filling; DNA replication; DNA catabolic process, exonucleolytic; telomere maintenance; response to UV

Disease: Colorectal Cancer, Susceptibility To, 10; Mandibular Hypoplasia, Deafness, Progeroid Features, And Lipodystrophy Syndrome