Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dipeptidyl peptidase 8 (DPP8) Standard Curve.)
Human Dipeptidyl Peptidase 8 (DPP8) ELISA Kit | DPP8 elisa kit
Human Dipeptidyl Peptidase 8 (DPP8) ELISA Kit
Gene Names
DPP8; DP8; DPRP1; DPRP-1; MST097; MSTP097; MSTP135; MSTP141
Reactivity
Human
Synonyms
Dipeptidyl Peptidase 8 (DPP8); Human Dipeptidyl Peptidase 8 (DPP8) ELISA Kit; DPP8; DP8; DPRP1; FLJ14920; FLJ20283; MGC26191; MSTP141; dipeptidyl peptidase 8; dipeptidyl peptidase IV-related protein-1; dipeptidyl peptidase VIII; dipeptidylpeptidase 8; prolyl dipeptidase DPP8; DPP8 elisa kit
Reactivity
Human
Specificity
Human Dipeptidyl peptidase 8 (DPP8) ELISA Kit has high sensitivity and excellent specificity for detection of Human DPP8. No significant cross-reactivity or interference between Human DPP8 and analogues was observed.
Sequence Length
847
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dipeptidyl peptidase 8 (DPP8) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dipeptidyl peptidase 8 (DPP8) Standard Curve.)
Related Product Information for DPP8 elisa kit
Description: This Human Dipeptidyl peptidase 8 (DPP8) ELISA Kit employs a two-site sandwich ELISA to quantitate DPP8 in samples. An antibody specific for DPP8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPP8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPP8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Dipeptidyl peptidase 8 is member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described.The deduced 882-amino acid DPP8 protein shares about 27% sequence identity with DPP4 and FAP; unlike these proteins, however, DPP8 has no transmembrane domain and no N- or O-linked glycosylation sites.
Background: Dipeptidyl peptidase 8 is member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described.The deduced 882-amino acid DPP8 protein shares about 27% sequence identity with DPP4 and FAP; unlike these proteins, however, DPP8 has no transmembrane domain and no N- or O-linked glycosylation sites.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dipeptidyl peptidase 8 isoform 4
NCBI Official Synonym Full Names
dipeptidyl peptidase 8
NCBI Official Symbol
DPP8
NCBI Official Synonym Symbols
DP8; DPRP1; DPRP-1; MST097; MSTP097; MSTP135; MSTP141
NCBI Protein Information
dipeptidyl peptidase 8
UniProt Protein Name
Dipeptidyl peptidase 8
Protein Family
UniProt Gene Name
DPP8
UniProt Synonym Gene Names
DPRP1; DP8; DPRP-1; DPP VIII
NCBI Description
This gene encodes a member of the peptidase S9B family, a small family of dipeptidyl peptidases that are able to cleave peptide substrates at a prolyl bond. The encoded protein shares similarity with dipeptidyl peptidase IV in that it is ubiquitously expressed, and hydrolyzes the same substrates. These similarities suggest that, like dipeptidyl peptidase IV, this protein may play a role in T-cell activation and immune function. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jul 2008]
Uniprot Description
Dipeptidyl peptidase that cleaves off N-terminal dipeptides from proteins having a Pro or Ala residue at position 2. May play a role in T-cell activation and immune function.
Research Articles on DPP8
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Product Notes
The Human DPP8 dpp8 (Catalog #AAA9715646) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715646 ELISA Kit recognizes Human DPP8. It is sometimes possible for the material contained within the vial of "Dipeptidyl Peptidase 8 (DPP8), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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