Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dipeptidyl peptidase 4 (DPP4) Standard Curve.)
Human Dipeptidyl Peptidase 4 (DPP4) ELISA Kit | DPP4 elisa kit
Human Dipeptidyl Peptidase 4 (DPP4) ELISA Kit
Gene Names
DPP4; CD26; ADABP; ADCP2; DPPIV; TP103
Reactivity
Human
Synonyms
Dipeptidyl Peptidase 4 (DPP4); Human Dipeptidyl Peptidase 4 (DPP4) ELISA Kit; DPP4; ADABP; ADCP2; CD26; DPPIV; TP103; T-cell activation antigen CD26; adenosine deaminase complexing protein 2; dipeptidylpeptidase 4; dipeptidylpeptidase IV; dipeptidylpeptidase IV (CD26; adenosine deamina; DPP4 elisa kit
Reactivity
Human
Specificity
Human Dipeptidyl peptidase 4 (DPP4) ELISA Kit has high sensitivity and excellent specificity for detection of Human DPP4. No significant cross-reactivity or interference between Human DPP4 and analogues was observed.
Sequence Length
766
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dipeptidyl peptidase 4 (DPP4) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Dipeptidyl peptidase 4 (DPP4) Standard Curve.)
Related Product Information for DPP4 elisa kit
Description: This Human Dipeptidyl peptidase 4 (DPP4) ELISA Kit employs a two-site sandwich ELISA to quantitate DPP4 in samples. An antibody specific for DPP4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DPP4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DPP4 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DPP4 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: The protein encoded by the DPPIV gene is an antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPPIV plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1.Furthermore it appears to work as a suppressor in the development of cancer and tumours.CD26/DPPIV plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum being increased in some neoplasms and decreased in others.
Background: The protein encoded by the DPPIV gene is an antigenic enzyme expressed on the surface of most cell types and is associated with immune regulation, signal transduction and apoptosis. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. DPPIV plays a major role in glucose metabolism. It is responsible for the degradation of incretins such as GLP-1.Furthermore it appears to work as a suppressor in the development of cancer and tumours.CD26/DPPIV plays an important role in tumor biology, and is useful as a marker for various cancers, with its levels either on the cell surface or in the serum being increased in some neoplasms and decreased in others.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
dipeptidyl peptidase 4
NCBI Official Synonym Full Names
dipeptidyl peptidase 4
NCBI Official Symbol
DPP4
NCBI Official Synonym Symbols
CD26; ADABP; ADCP2; DPPIV; TP103
NCBI Protein Information
dipeptidyl peptidase 4
UniProt Protein Name
Dipeptidyl peptidase 4
Protein Family
UniProt Gene Name
DPP4
UniProt Synonym Gene Names
ADCP2; CD26; ADCP-2; DPP IV
UniProt Entry Name
DPP4_HUMAN
NCBI Description
The protein encoded by this gene is identical to adenosine deaminase complexing protein-2, and to the T-cell activation antigen CD26. It is an intrinsic membrane glycoprotein and a serine exopeptidase that cleaves X-proline dipeptides from the N-terminus of polypeptides. [provided by RefSeq, Jul 2008]
Research Articles on DPP4
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Product Notes
The Human DPP4 dpp4 (Catalog #AAA9715648) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715648 ELISA Kit recognizes Human DPP4. It is sometimes possible for the material contained within the vial of "Dipeptidyl Peptidase 4 (DPP4), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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