Human Dickkopf Related Protein 4 (DKK4) ELISA Kit | DKK4 elisa kit

Human Dickkopf Related Protein 4 (DKK4) ELISA Kit

Cat. Num. AAA450820

• Gene Names: DKK4; DKK-4
• Reactivity: Human
• Synonyms: Dickkopf Related Protein 4 (DKK4); Human Dickkopf Related Protein 4 (DKK4) ELISA Kit; DKK4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of DKK4. No significant cross-reactivity or interference between DKK4 and analogues was observed.
• Sequence Length: 224

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.067ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DKK4 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DKK4 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DKK4 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of DKK4 in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to DKK4. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to DKK4. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DKK4, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DKK4 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 7657025
• NCBI GeneID: 27121
• NCBI Accession #: NP_055235.1
• NCBI GenBank Nucleotide #: NM_014420.2
• UniProt Accession #: Q9UBT3; Q3KNX0; Q9Y4C3
• Molecular Weight: 24,876 Da
• NCBI Official Full Name: dickkopf-related protein 4
• NCBI Official Synonym Full Names: dickkopf WNT signaling pathway inhibitor 4
• NCBI Official Symbol: DKK4
• NCBI Official Synonym Symbols: DKK-4
• NCBI Protein Information: dickkopf-related protein 4
• UniProt Protein Name: Dickkopf-related protein 4
• Protein Family: Dickkopf-related protein
• UniProt Gene Name: DKK4
• UniProt Synonym Gene Names: Dickkopf-4; Dkk-4; hDkk-4

NCBI Description

This gene encodes a protein that is a member of the dickkopf family. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. [provided by RefSeq, Jul 2008]

Uniprot Description

Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease ().