Rabbit USP2 Polyclonal Antibody | anti-USP2 antibody

Rabbit Polyclonal to Human USP2

Cat. Num. AAA241432

• Gene Names: USP2; USP9; UBP41
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Dog, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: USP2; Polyclonal Antibody; Rabbit Polyclonal to Human USP2; Anti-USP2 Antibody (Internal) IHC-plus; Deubiquitinating enzyme 2; Ubiquitin specific peptidase 2; Ubiquitin specific protease 9; USP9; Ubiquitin specific protease 12; Ubiquitin specific protease 2; Ubiquitin thioesterase 2; UBP41; Human USP2; anti-USP2 antibody

• Host: Rabbit
• Reactivity: Gibbon, Gorilla, Human, Monkey; Predicted Reactivity: Dog, Horse, Pig, Rabbit (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human USP2. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 605

• Applicable Applications for anti-USP2 antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (1.25 - 2.5 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic 18 amino acid peptide from internal region of human USP2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Dog, Panda, Horse, Rabbit, Pig (94%).
• Immunogen Type: Synthetic peptide
• Immunogen: USP2 antibody was raised against synthetic 18 amino acid peptide from internal region of human USP2. Percent identity with other species by BLAST analysis: Human, Gorilla, Gibbon, Monkey (100%); Dog, Panda, Horse, Rabbit, Pig (94%); Marmoset, Mouse, Rat, Bovine, Hamster, Elephant (89%); Opossum (83%).
• Antigen Modification: Internal
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-USP2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-USP2 antibody

UBP, an ubiquitin-specific protease, is selectively up regulated in bone by the osteotropic agents PTH, PTHrP and PGE2 and possibly via the PKA/cAMP pathway. It is also thought to play a role in the recycling of ubiquitin by hydrolysis of branched poly-ubiquitin from linear poly-ubiquitin chains, production of free ubiquitin from linear poly-ubiquitin chains and from certain ribosomal ubiquitin fusion proteins.

Product Categories/Family for anti-USP2 antibody

Family: Protease; Subfamily: Cysteine C19

NCBI and Uniprot Product Information

• NCBI GI #: 188528692
• NCBI GeneID: 9099
• NCBI Accession #: NP_004196.4
• NCBI GenBank Nucleotide #: NM_004205.4
• UniProt Accession #: O75604; Q8IUM2; Q8IW04; Q96MB9; Q9BQ21; B0YJB8; E9PPM2
• Molecular Weight: 45,241 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase 2 isoform a
• NCBI Official Synonym Full Names: ubiquitin specific peptidase 2
• NCBI Official Symbol: USP2
• NCBI Official Synonym Symbols: USP9; UBP41
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase 2; ubiquitin thioesterase 2; deubiquitinating enzyme 2; ubiquitin specific protease 9; ubiquitin specific protease 12; 41 kDa ubiquitin-specific protease; ubiquitin-specific-processing protease 2
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase 2
• Protein Family: Protein
• UniProt Gene Name: USP2
• UniProt Synonym Gene Names: UBP41
• UniProt Entry Name: UBP2_HUMAN

NCBI Description

This gene encodes a member of the family of de-ubiquitinating enzymes, which belongs to the peptidase C19 superfamily. The encoded protein is a ubiquitin-specific protease which is required for TNF-alpha (tumor necrosis factor alpha) -induced NF-kB (nuclear factor kB) signaling. This protein deubiquitinates polyubiquitinated target proteins such as fatty acid synthase, murine double minute 2 (MDM2), MDM4/MDMX and cyclin D1. MDM2 and MDM4 are negative regulators of the p53 tumor suppressor and cyclin D1 is required for cell cycle G1/S transition. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]

Uniprot Description

USP2: Hydrolase that deubiquitinates polyubiquitinated target proteins such as MDM2, MDM4 and CCND1. Isoform 1 and isoform 4 possess both ubiquitin-specific peptidase and isopeptidase activities. Deubiquitinates MDM2 without reversing MDM2-mediated p53/TP53 ubiquitination and thus indirectly promotes p53/TP53 degradation and limits p53 activity. Has no deubiquitinase activity against p53/TP53. Prevents MDM2-mediated degradation of MDM4. Plays a role in the G1/S cell-cycle progression in normal and cancer cells. Plays a role in the regulation of myogenic differentiation of embryonic muscle cells. Homooligomer. Found in trimeric complex with MDM2 and MDM4 and UPB2. Interacts with CCND1; the interaction is direct and promotes its stabilization by antagonizing ubiquitin-dependent degradation. Interacts (via N-terminus and C- terminus) with MDM2. Interacts with MDM4. Down-regulated by cisplatin. Expressed in mesangial cells of the kidney and in different types of glomerulonephritides. Cleavage is inhibited by ubiquitin in a dosage- dependent manner. Cleavage is blocked by ubiquitin aldehyde. Belongs to the peptidase C19 family. USP2 subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Ubiquitin-specific protease; EC 3.4.19.12

Chromosomal Location of Human Ortholog: 11q23.3

Cellular Component: centrosome; perinuclear region of cytoplasm; cytoplasm; cell cortex; nucleus

Molecular Function: identical protein binding; protein binding; cyclin binding; ubiquitin protein ligase binding; metal ion binding; cysteine-type endopeptidase activity; ubiquitin-specific protease activity

Biological Process: locomotor rhythm; proteasomal ubiquitin-dependent protein catabolic process; negative regulation of skeletal muscle development; muscle development; protein deubiquitination; protein stabilization; positive regulation of skeletal muscle development; negative regulation of transcription from RNA polymerase II promoter; circadian regulation of gene expression; positive regulation of mitotic cell cycle; cell cycle; circadian behavior; entrainment of circadian clock by photoperiod