Typical Testing Data/Standard Curve (for reference only)
Human Dicer 1, Ribonuclease Type III (DICER1) ELISA Kit | DICER1 elisa kit
Human Dicer 1, Ribonuclease Type III (DICER1) ELISA Kit
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: The microplate provided in this kit has been pre-coated with an antibody specific to DICER1. Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to DICER1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DICER1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of DICER1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein possessing an RNA helicase motif containing a DEXH box in its amino terminus and an RNA motif in the carboxy terminus. The encoded protein functions as a ribonuclease and is required by the RNA interference and small temporal RNA (stRNA) pathways to produce the active small RNA component that represses gene expression. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2010]
Uniprot Description
DICER1: a cytoplasmic type-III RNase that plays a central role in RNA interference (RNAi) pathways to produce the active small RNAs that represses gene expression. Possesses a DEAD box RNA helicase motif in its amino terminus and a dsRNA-binding motif in the carboxy terminus. Dicer, along with Ago2 andTRBP, are the main components of the RNA-induced silencing complex (RISC). Fragile X syndrome repeats form RNA hairpins that are cut by Dicer but do not activate the interferon-inducible protein kinase, PKR. Four alternatively spliced isoforms have been described.
Protein type: Ribonuclease; EC 3.1.26.3; Cell development/differentiation; RNA-binding; Helicase; RNA processing
Chromosomal Location of Human Ortholog: 14q32.13
Cellular Component: growth cone; axon; cytoplasm; dendrite; ER-Golgi intermediate compartment; nucleus; cytosol
Molecular Function: endoribonuclease activity; protein binding; miRNA binding; ribonuclease III activity; metal ion binding; double-stranded RNA binding; deoxyribonuclease I activity; helicase activity; ATP binding
Biological Process: zygote asymmetric cell division; regulation of cell cycle; regulation of neuron differentiation; regulation of oligodendrocyte differentiation; miRNA-mediated gene silencing, miRNA loading onto RISC; negative regulation of transcription from RNA polymerase II promoter; embryonic hindlimb morphogenesis; olfactory bulb interneuron differentiation; post-embryonic development; cardiac muscle cell development; pre-microRNA processing; angiogenesis; DNA fragmentation during apoptosis; defense response to virus; regulation of viral genome replication; positive regulation of myelination; spleen development; positive regulation of Schwann cell differentiation; hair follicle morphogenesis; inner ear receptor cell development; mRNA stabilization; multicellular organism growth; RNA interference, targeting of mRNA for destruction; stem cell maintenance; RNA interference, conversion of ds siRNA to ss siRNA; spindle assembly; spinal cord motor neuron differentiation; miRNA-mediated gene silencing, production of miRNAs; myelin formation in the peripheral nervous system; branching morphogenesis of a tube; cartilage development; nerve development; gene expression; cerebral cortex development; reproductive structure development; RNA interference, production of siRNA; neurite morphogenesis; lung development; myoblast cell differentiation involved in skeletal muscle regeneration
Disease: Pleuropulmonary Blastoma; Goiter, Multinodular 1, With Or Without Sertoli-leydig Cell Tumors; Rhabdomyosarcoma, Embryonal, 2
Research Articles on DICER1
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Product Notes
The Human DICER1 dicer1 (Catalog #AAA2021899) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2021899 ELISA Kit recognizes Human DICER1. It is sometimes possible for the material contained within the vial of "Dicer 1, Ribonuclease Type III (DICER1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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