Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with POTE antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Rabbit anti-Human POTE Polyclonal Antibody | anti-POTED antibody
POTE Antibody (C-term)
Reactivity
Human
Applications
ELISA, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
POTE; Polyclonal Antibody; POTE Antibody (C-term); POTE ankyrin domain family member D; ANKRD26-like family B member 3; Ankyrin repeat domain-containing protein 21; Prostate; ovary; testis-expressed protein; Protein POTE; POTED; A26B3; ANKRD21; anti-POTED antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This POTE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 542-571 amino acids from the C-terminal region of human POTE.
Purity/Purification
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Positions
542-571
Sequence Length
584
Applicable Applications for anti-POTED antibody
ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC~~1:10~50
WB~~1:1000
WB~~1:1000
Antigen Type
Synthetic Peptide
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with POTE antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with POTE antibody (C-term) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Western Blot (WB)
(Western blot analysis of POTE Antibody (C-term) in Ramos cell line lysates (35ug/lane). POTE (arrow) was detected using the purified Pab.)
Western Blot (WB)
(Western blot analysis of POTE Antibody (C-term) in Ramos cell line lysates (35ug/lane). POTE (arrow) was detected using the purified Pab.)
Related Product Information for anti-POTED antibody
The POTE protein contains seven ankyrin repeats between amino acids 140 and 380. Expression of POTE in prostate cancer and its undetectable expression in normal essential tissues make POTE a candidate for the immunotherapy of prostate cancer. The existence of a large number of closely related but rapidly diverging members, their location on multiple chromosomes and their limited expression pattern suggest an important role for the POTE gene family in reproductive processes.
Product Categories/Family for anti-POTED antibody
References
Bera T.K., Proc. Natl. Acad. Sci. U.S.A. 99:16975-16980(2002).
Bera,T.K., Gene 337, 45-53 (2004)
Bera,T.K., Gene 337, 45-53 (2004)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
66394
NCBI Official Full Name
uncharacterized protein LOC100288966
NCBI Official Symbol
LOC100288966
NCBI Protein Information
uncharacterized protein LOC100288966
UniProt Protein Name
POTE ankyrin domain family member D
Protein Family
UniProt Gene Name
POTED
UniProt Synonym Gene Names
A26B3; ANKRD21; POTE; Protein POTE
UniProt Entry Name
POTED_HUMAN
Uniprot Description
ANKRD21: a peripheral membrane protein expressed in prostate, ovary, testis, placenta and prostate cancer cell lines.
Protein type: Cancer Testis Antigen (CTA)
Chromosomal Location of Human Ortholog: 21q11.2
Cellular Component: plasma membrane
Similar Products
Product Notes
The POTED poted (Catalog #AAA9205349) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 542-571. The POTE Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's POTE can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB). IHC~~1:10~50 WB~~1:1000. Researchers should empirically determine the suitability of the POTED poted for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "POTE, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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