anti-Transfected GADD45alpha Monoclonal Antibody | anti-GADD45alpha antibody

Anti-GADD45alpha Mouse mAb

Cat. Num. AAA475149

• Gene Names: GADD45A; DDIT1; GADD45
• Reactivity: Transfected
• Applications: Western Blot
• Purity: Affinity Purified
• Synonyms: GADD45alpha; Monoclonal Antibody; Anti-GADD45alpha Mouse mAb; DDIT 1; DDIT-1; DDIT1; DNA damage inducible transcript 1; DNA damage-inducible transcript 1 protein; GA45A_HUMAN; GADD45; GADD45A; Growth arrest and DNA damage inducible 45 alpha; Growth arrest and DNA damage inducible alpha; Growth arrest and DNA damage-inducible protein GADD45 alpha; anti-GADD45alpha antibody

• Host: Mouse; Source: Human
• Reactivity: Transfected
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 6C3-G3-E9
• Purity/Purification: Affinity Purified
• Form/Format: Purified mouse monoclonal antibody in PBS (pH 7.4) containing with 0.02% sodium azide and 50% glycerol.
• Concentration: 4.4mg/ml (varies by lot)
• Sequence Length: 131

• Applicable Applications for anti-GADD45alpha antibody: Western Blot (WB)
• Application Notes: WB: 1:1000
• Immunogen: Purified recombinant human GADD45 alpha protein expressed in E Coli
• Sensitivity: Transfected Only.
• Sequence Similarities: Belongs to the GADD45 family.
• Subunit Structure: Interacts with MAPK14. Predominantly monomeric but also forms dimers and other oligomers as concentration increases. Interacts with GADD45GIP1. Interacts weakly with PCNA. Interacts with AURKA, likely to compete with dimerization.
• Subcellular Location: Nucleus.

Western Blot (WB)

(Western blot detection of GADD45 alpha in CHO-K1 cell lysate (B)and CHO-K1 transfected by GADD45 alpha His fusion protein (A)cell lysate using GADD45 alpha mouse mAb (1:1000 diluted). Predicted band size:22KDa. Observed band size:22KDa.)

Related Product Information for anti-GADD45alpha antibody

Entrez Summary: This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. (provided by RefSeq, Dec 2010).

Product Categories/Family for anti-GADD45alpha antibody

Epigenetics and Nuclear Signaling; DNA/RNA; DNA Damage & Repair; Base Excision Repair

NCBI and Uniprot Product Information

• NCBI GI #: 315075323
• NCBI GeneID: 1647
• NCBI Accession #: NP_001186670.1
• NCBI GenBank Nucleotide #: NM_001924
• UniProt Accession #: P24522; Q5TCA7; Q5TCA8
• Molecular Weight: 22
• NCBI Official Full Name: growth arrest and DNA damage-inducible protein GADD45 alpha isoform 2
• NCBI Official Synonym Full Names: growth arrest and DNA damage inducible alpha
• NCBI Official Symbol: GADD45A
• NCBI Official Synonym Symbols: DDIT1; GADD45
• NCBI Protein Information: growth arrest and DNA damage-inducible protein GADD45 alpha
• UniProt Protein Name: Growth arrest and DNA damage-inducible protein GADD45 alpha
• UniProt Gene Name: GADD45A
• UniProt Synonym Gene Names: DDIT1; GADD45; DDIT-1

NCBI Description

This gene is a member of a group of genes whose transcript levels are increased following stressful growth arrest conditions and treatment with DNA-damaging agents. The protein encoded by this gene responds to environmental stresses by mediating activation of the p38/JNK pathway via MTK1/MEKK4 kinase. The DNA damage-induced transcription of this gene is mediated by both p53-dependent and -independent mechanisms. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene.[provided by RefSeq, Dec 2010]

Uniprot Description

GADD45A: In T-cells, functions as a regulator of p38 MAPKs by inhibiting p88 phosphorylation and activity. Might affect PCNA interaction with some CDK (cell division protein kinase) complexes; stimulates DNA excision repair in vitro and inhibits entry of cells into S phase. Interacts with MAPK14. Interacts with GADD45GIP1. Interacts with PCNA. By UV irradiation, X-rays, growth arrest and alkylating agents. The induction is mediate by some kinase(s) other than PKC. Belongs to the GADD45 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Apoptosis; Cell cycle regulation; DNA repair, damage; Nuclear receptor co-regulator

Chromosomal Location of Human Ortholog: 1p31.3

Cellular Component: cytoplasm; nucleoplasm; nucleus

Molecular Function: protein binding

Biological Process: activation of MAPKKK activity; apoptosis; cell cycle arrest; DNA damage response, signal transduction; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; DNA repair; positive regulation of apoptosis; positive regulation of JNK cascade; regulation of cyclin-dependent protein kinase activity