Human Dengue Virus IgM ELISA Kit
Dengue Virus IgM ELISA Kit
Reactivity
Human
Synonyms
Dengue Virus IgM; Dengue Virus IgM ELISA Kit; Dengue Virus IgM ELISA; Dengue Virus IgM elisa kit
Reactivity
Human
Preparation and Storage
Product should be stored at 2-8 degree C. Product is stable for 24 months from the date of manufacturing.
Related Product Information for Dengue Virus IgM elisa kit
Intended Uses: Dengue virus IgG ELISA Kit is intended for the detection of IgG antibody to Dengue virus in human serum or plasma. For research use only.
Principle of the Assay: Diluted patient serum and biotinylated anti-human IgG antibody reagent are added to the wells coated with Streptavidin. IgG antibodies in the patient serum binds to the x-h IgG antibody, and the complex is immobilized on the plate through streptavidin-biotin interactions. Unbound proteins are washed off through a washing step. Dengue antigen-HRP conjugate reagent is added into the wells. Dengue virus IgG specific antibody, if present, binds to the antigen. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the oxidation of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.
Principle of the Assay: Diluted patient serum and biotinylated anti-human IgG antibody reagent are added to the wells coated with Streptavidin. IgG antibodies in the patient serum binds to the x-h IgG antibody, and the complex is immobilized on the plate through streptavidin-biotin interactions. Unbound proteins are washed off through a washing step. Dengue antigen-HRP conjugate reagent is added into the wells. Dengue virus IgG specific antibody, if present, binds to the antigen. Excess enzyme conjugate is washed off and substrate is added. The plate is incubated to allow the oxidation of the substrate by the enzyme. The intensity of the color generated is proportional to the amount of IgG specific antibody in the sample.
Product Categories/Family for Dengue Virus IgM elisa kit
References
1. Pinheiro FP, Corber SJ: Global situation of dengue and dengue haemorrhagic fever, and its emergence in the Americas. World Health Stat Q 50(3/4):161-169, 1997. 2. Gubler DJ, Trent DW: Emergence of epidemic dengue/dengue hemorrhagic fever as a public health problem in the Americas. Infect Agents Dis 2:383-393, 1993. 3. Wu SJ; Hanson B; Paxton H; Nisalak A; Vaughn DW; Rossi C; Henchal EA; Porter KR; Watts DM; Hayes CG. Evaluation of a dipstick enzyme-linked immunosorbent assay for detection of antibodies to dengue virus. Clin Diagn Lab Immunol1997; 4(4):452-7.4. Lam SK; Devine PL. Evaluation of capture ELISA and rapid immunochromatographic test for the determination of IgM and IgG antibodies produced during dengue infection. Clin Diagn Virol 1998;10(1):75-8. 5. Rossi CA; Drabick JJ; Gambel JM; Sun W; Lewis TE; Henchal EA. Laboratory diagnosis of acute dengue fever during the United Nations Mission in Haiti, 1995-1996. Am J Trop Med Hyg 1998;59(2):275-8
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Product Notes
The Human Dengue Virus IgM (Catalog #AAA580101) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA580101 ELISA Kit recognizes Human Dengue Virus IgM. It is sometimes possible for the material contained within the vial of "Dengue Virus IgM, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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