Human Defensin Alpha 4, Corticostatin (DEFa4) ELISA Kit | DEFa4 elisa kit

Human Defensin Alpha 4, Corticostatin (DEFa4) ELISA Kit

Cat. Num. AAA2020962

• Gene Names: DEFA4; HP4; DEF4; HP-4; HNP-4
• Reactivity: Human
• Synonyms: Defensin Alpha 4; Corticostatin (DEFa4); Human Defensin Alpha 4; Corticostatin (DEFa4) ELISA Kit; DEF4; HP-4; HNP-4; Neutrophil defensin 4; DEFa4 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Defensin Alpha 4, Corticostatin (DEFa4).; No significant cross-reactivity or interference between Defensin Alpha 4, Corticostatin (DEFa4) and analogues was observed.
• Sequence Length: 97

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 31.25-2000pg/mL
• Sensitivity: < 13.8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Defensin Alpha 4, Corticostatin (DEFa4) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Defensin Alpha 4, Corticostatin (DEFa4) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DEFa4 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Defensin Alpha 4, Corticostatin (DEFa4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Defensin Alpha 4, Corticostatin (DEFa4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Defensin Alpha 4, Corticostatin (DEFa4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Defensin Alpha 4, Corticostatin (DEFa4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for DEFa4 elisa kit

Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 50057841
• NCBI GeneID: 1669
• UniProt Accession #: P12838; Q6EZF8
• Molecular Weight: 10,504 Da
• NCBI Official Full Name: defensin, alpha 4, corticostatin
• NCBI Official Synonym Full Names: defensin, alpha 4, corticostatin
• NCBI Official Symbol: DEFA4
• NCBI Official Synonym Symbols: HP4; DEF4; HP-4; HNP-4
• NCBI Protein Information: neutrophil defensin 4
• UniProt Protein Name: Neutrophil defensin 4
• Protein Family: Alpha-defensin
• UniProt Gene Name: DEFA4
• UniProt Synonym Gene Names: DEF4; HP-4
• UniProt Entry Name: DEF4_HUMAN

NCBI Description

Defensins are a family of antimicrobial and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. Several alpha defensin genes are clustered on chromosome 8. This gene differs from other genes of this family by an extra 83-base segment that is apparently the result of a recent duplication within the coding region. The protein encoded by this gene, defensin, alpha 4, is found in the neutrophils; it exhibits corticostatic activity and inhibits corticotropin stimulated corticosterone production. [provided by RefSeq, Oct 2014]

Uniprot Description

DEFA4: Has antimicrobial activity against Gram-negative bacteria, and to a lesser extent also against Gram-positive bacteria and fungi. Protects blood cells against infection with HIV-1 (in vitro). Inhibits corticotropin (ACTH)-stimulated corticosterone production. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23

Cellular Component: extracellular space; Golgi lumen; extracellular region

Biological Process: killing of cells of another organism; antibacterial humoral response; innate immune response; defense response to fungus