Rabbit anti-Human TRAF3 Polyclonal Antibody | anti-TRAF3 antibody

TRAF3 (TNF Receptor-associated Factor 3, CAP1, LAP1, CAP-1, CRAF1, IIAE5, CD40bp) (HRP)

Cat. Num. AAA6441581

• Gene Names: TRAF3; CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
• Reactivity: Human
• Applications: Immunofluorescence, Western Blot, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: TRAF3; Polyclonal Antibody; TRAF3 (TNF Receptor-associated Factor 3; CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp) (HRP); anti-TRAF3 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human TRAF3.
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-TRAF3 antibody: Immunofluorescence (IF), Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: IF: 1:50-1:200; WB: 1:200-1:1000; IHC: 1:50-1:200; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to human TNF receptor-associated factor 3.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts of mouse kidney cell lines, using TRAF3 antibody.)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human tonsil tissue using TRAF3 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of HeLa cell using TRAF3 antibody. Blue: DAPI for nuclear staining.)

Product Categories/Family for anti-TRAF3 antibody

Antibodies; TRAF

NCBI and Uniprot Product Information

• NCBI GI #: 116242824
• NCBI GeneID: 7187
• UniProt Accession #: Q13114; Q12990; Q13076; Q13947; Q6AZX1; Q9UNL1; B7Z8C4
• Molecular Weight: 568
• NCBI Official Full Name: TNF receptor-associated factor 3
• NCBI Official Synonym Full Names: TNF receptor-associated factor 3
• NCBI Official Symbol: TRAF3
• NCBI Official Synonym Symbols: CAP1; LAP1; CAP-1; CRAF1; IIAE5; CD40bp
• NCBI Protein Information: TNF receptor-associated factor 3; CD40 binding protein; CD40 associated protein 1; LMP1-associated protein 1; CD40 receptor associated factor 1
• UniProt Protein Name: TNF receptor-associated factor 3
• Protein Family: TRAF3-interacting protein
• UniProt Gene Name: TRAF3
• UniProt Synonym Gene Names: CAP1; CRAF1; CRAF1; CD40BP; LAP1
• UniProt Entry Name: TRAF3_HUMAN

NCBI Description

The protein encoded by this gene is a member of the TNF receptor associated factor (TRAF) protein family. TRAF proteins associate with, and mediate the signal transduction from, members of the TNF receptor (TNFR) superfamily. This protein participates in the signal transduction of CD40, a TNFR family member important for the activation of the immune response. This protein is found to be a critical component of the lymphotoxin-beta receptor (LTbetaR) signaling complex, which induces NF-kappaB activation and cell death initiated by LTbeta ligation. Epstein-Barr virus encoded latent infection membrane protein-1 (LMP1) can interact with this and several other members of the TRAF family, which may be essential for the oncogenic effects of LMP1. Several alternatively spliced transcript variants encoding three distinct isoforms have been reported. [provided by RefSeq, Dec 2010]

Uniprot Description

TRAF3: Regulates pathways leading to the activation of NF- kappa-B and MAP kinases, and plays a central role in the regulation of B-cell survival. Part of signaling pathways leading to the production of cytokines and interferon. Required for normal antibody isotype switching from IgM to IgG. Plays a role T-cell dependent immune responses. Plays a role in the regulation of antiviral responses. Is an essential constituent of several E3 ubiquitin-protein ligase complexes. May have E3 ubiquitin-protein ligase activity and promote 'Lys-63'-linked ubiquitination of target proteins. Inhibits activation of NF-kappa-B in response to LTBR stimulation. Inhibits TRAF2-mediated activation of NF-kappa- B. Down-regulates proteolytic processing of NFKB2, and thereby inhibits non-canonical activation of NF-kappa-B. Promotes ubiquitination and proteasomal degradation of MAP3K14. Homotrimer. Heterotrimer with TRAF2 and TRAF5. Interacts with LTBR/TNFRSF3, TNFRSF4, TNFRSF5/CD40, TNFRSF8/CD30, TNFRSF13C TNFRSF17/BCMA, TLR4 and EDAR. Interacts with MAP3K5, MAP3K14, TRAIP/TRIP, TDP2/TTRAP, TANK/ITRAF and TRAF3IP1. Interaction with TNFRSF5/CD40 is modulated by TANK/ITRAF, which competes for the same binding site. Interacts with TICAM1. Interacts with TRAFD1. Interacts with OTUB1, OTUB2 and OTUD5. Interacts with RNF216, MAVS, OPTN and TBK1. Identified in a complex with TRAF2, MAP3K14 and BIRC3. Interacts with BIRC2 and BIRC3. Upon exposure to bacterial lipopolysaccharide (LPS), recruited to a transient complex containing TLR4, TRAF3, TRAF6, IKBKG, MAP3K7, MYD88, TICAM1, BIRC2, BIRC3 and UBE2N. Interacts with Epstein-Barr virus protein LMP1. Interacts (via RING-type zinc finger domain) with SRC. Belongs to the TNF receptor-associated factor family. A subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 14q32.32

Cellular Component: internal side of plasma membrane; mitochondrion; cytosol; endosome

Molecular Function: protein binding; signal transducer activity; zinc ion binding; thioesterase binding; ubiquitin protein ligase binding; ubiquitin-protein ligase activity; tumor necrosis factor receptor binding; protein kinase binding; ligase activity

Biological Process: apoptosis; MyD88-independent toll-like receptor signaling pathway; regulation of defense response to virus; protein ubiquitination; toll-like receptor 3 signaling pathway; signal transduction; Toll signaling pathway; regulation of cytokine production; regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; inhibition of NF-kappaB transcription factor; regulation of proteolysis; toll-like receptor signaling pathway; innate immune response; regulation of interferon-beta production; toll-like receptor 4 signaling pathway; negative regulation of interferon type I production

Disease: Herpes Simplex Encephalitis, Susceptibility To, 3