Human DAND5/COCO ELISA Kit | DAND5/COCO elisa kit

Human DAND5/COCO PicoKine ELISA Kit

Cat. Num. AAA1751577

• Gene Names: DAND5; SP1; CER2; COCO; CRL2; CERL2; DANTE; GREM3; CKTSF1B3
• Reactivity: Human
• Synonyms: DAND5/COCO; Human DAND5/COCO PicoKine ELISA Kit; Human DAND5; DAND5/COCO elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human DAND5
• Sequence Length: 189

• Samples: Cell Culture Supernates, Serum And Plasma(Heparin, Edta, Citrate)
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay): Three samples of known concentration were tested on one plate to assess intra-assay precision
• Inter-assay Precision: Inter-Assay Precision (Precision accross assays):Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DAND5/COCO elisa kit

Principle of the Assay: The Picokine Human CXCL8 Pre-Coated ELISA (Enzyme-Linked Immunosorbent Assay) kit is a solid phase immunoassay specially designed to measure Human CXCL8 with a 96-well strip plate that is pre-coated with antibody specific for CXCL8. The detection antibody is a biotinylated antibody specific for CXCL8. The capture antibody is monoclonal antibody from mouse, the detection antibody is polyclonal antibody from goat. The kit contains recombinant Human CXCL8 with immunogen: Expression system for standard: E.coli; Immunogen sequence: S28-S99. The kit is analytically validated with ready to use reagents. To measure Human CXCL8, add standards and samples to the wells, then add the biotinylated detection antibody. Wash the wells with PBS or TBS buffer, and add Avidin-Biotin-Peroxidase Complex (ABC-HRP). Wash away the unbounded ABC-HRP with PBS or TBS buffer and add TMB. TMB is substrate to HRP and will be catalyzed to produce a blue color product, which changes into yellow after adding acidic stop solution. The density of the yellow product is linearly propotional to Human CXCL8 in the sample. Read the density of the yellow product in each well using a plate reader, and benchmark the sample wells' readings against the standard curve to determine the concentration of Human CXCL8 in the sample!!Background/Introduction: Interleukin-8, also called neutrophil-activating peptide-1 or SCYB8, is a tissue-derived peptide secreted by several types of cells in response to inflammatory stimuli. Monocyte-derived neutrophil chemotactic factor(MDNCF/IL-8, suggested gene symbol IL8) is a cytokine that chemoattracts and activates neutrophils. IL-8 is produced and released from human adipose tissue and from isolated adipocytes in vitro, which may indicate that IL-8 from adipose tissue could be involved in some of the obesity-related complications. The MDNCF/IL-8 gene is placed on the human gene map at position 4q12-q21. This is the same location where at least three other members(platelet factor 4, melanoma growth stimulatory activity, and interferon-gamma induced factor) of the platelet factor 4 gene superfamily reside. Human IL-8 consists of 99 amino acids in precursor form and 79 amino acids in mature form.

NCBI and Uniprot Product Information

• NCBI GI #: 22749329
• NCBI GeneID: 199699
• NCBI Accession #: NP_689867.1
• NCBI GenBank Nucleotide #: NM_152654.2
• NCBI Official Full Name: DAN domain family member 5
• NCBI Official Synonym Full Names: DAN domain BMP antagonist family member 5
• NCBI Official Symbol: DAND5
• NCBI Official Synonym Symbols: SP1; CER2; COCO; CRL2; CERL2; DANTE; GREM3; CKTSF1B3
• NCBI Protein Information: DAN domain family member 5

NCBI Description

This gene encodes a member of the BMP (bone morphogenic protein) antagonist family. Like BMPs, BMP antagonists contain cystine knots and typically form homo- and heterodimers. The CAN (cerberus and dan) subfamily of BMP antagonists, to which this gene belongs, is characterized by a C-terminal cystine knot with an eight-membered ring. The antagonistic effect of the secreted protein encoded by this gene is likely due to its direct binding to BMP proteins. As an antagonist of BMP, this gene may play a role in regulating organogenesis, body patterning, and tissue differentiation. In mouse, this protein has been shown to bind Nodal and to inhibit the Nodal signaling pathway which patterns left/right body asymmetry. [provided by RefSeq, Jul 2008]