Human Cytochrome P450 4F2 (CYP4F2) ELISA Kit | CYP4F2 elisa kit

Human Cytochrome P450 4F2 (CYP4F2) ELISA Kit

Cat. Num. AAA2706928

• Gene Names: CYP4F2; CPF2
• Reactivity: Human
• Synonyms: Cytochrome P450 4F2 (CYP4F2); Human Cytochrome P450 4F2 (CYP4F2) ELISA Kit; CPF2; Leukotriene-B(4)omega-hydroxylase 1; Cytochrome P450 Family 4 Subfamily F Polypeptide 2; 20-hydroxyeicosatetraenoic acid synthase; Arachidonic acid omega-hydroxylase; CYP4F2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 4F2 (CYP4F2).; No significant cross-reactivity or interference between Cytochrome P450 4F2 (CYP4F2) and analogues was observed.
• Sequence Length: 190

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 1.56-100ng/mL
• Sensitivity: < 0.55ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 4F2 (CYP4F2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 4F2 (CYP4F2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP4F2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 4F2 (CYP4F2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 4F2 (CYP4F2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 4F2 (CYP4F2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 4F2 (CYP4F2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 9313018
• NCBI GeneID: 8529
• Molecular Weight: 21,709 Da
• NCBI Official Full Name: cytochrome P450 4F2
• NCBI Official Synonym Full Names: cytochrome P450 family 4 subfamily F member 2
• NCBI Official Symbol: CYP4F2
• NCBI Official Synonym Symbols: CPF2
• NCBI Protein Information: phylloquinone omega-hydroxylase CYP4F2
• UniProt Protein Name: Phylloquinone omega-hydroxylase CYP4F2
• Protein Family: Phylloquinone omega-hydroxylase
• UniProt Gene Name: CYP4F2
• UniProt Synonym Gene Names: 20-HETE synthase

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F11, is approximately 16 kb away. [provided by RefSeq, Jul 2008]

Uniprot Description

Omega-hydroxylase that oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids and xenobiotics. Plays a key role in vitamin K catabolism by mediating omega-hydroxylation of vitamin K1 (phylloquinone), and menaquinone-4 (MK-4), a form of vitamin K2. Hydroxylation of phylloquinone and MK-4 probably regulates blood coagulation (PubMed:19297519, PubMed:24138531). Also shows arachidonic acid omega-hydroxylase activity in kidney, by mediating conversion of arachidonic acid to 20-hydroxyeicosatetraenoic acid (20-HETE), possibly influencing blood pressure control (PubMed:10660572, PubMed:17341693, PubMed:18574070). Also acts as a leukotriene-B4 omega-hydroxylase by mediating conversion of leukotriene-B4 (LTB4) to its omega-hydroxylated metabolite 20-hydroxyleukotriene-B4 (20-OH LTB4) (PubMed:8026587, PubMed:9799565).