Human Cytochrome P450 2C9 (CYP2C9) ELISA Kit | CYP2C9 elisa kit

Human Cytochrome P450 2C9 (CYP2C9) ELISA Kit

Cat. Num. AAA2025121

• Gene Names: CYP2C9; CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
• Reactivity: Human
• Synonyms: Cytochrome P450 2C9 (CYP2C9); Human Cytochrome P450 2C9 (CYP2C9) ELISA Kit; CYP2C10; P450IIC9; Cytochrome P450; Subfamily IIC(Mephenytoin 4-Hydroxylase)Polypeptide 9; S-mephenytoin 4-hydroxylase; CYP2C9 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cytochrome P450 2C9 (CYP2C9).; No significant cross-reactivity or interference between Cytochrome P450 2C9 (CYP2C9) and analogues was observed.
• Sequence Length: 490

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.625-40ng/mL
• Sensitivity: < 0.236ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cytochrome P450 2C9 (CYP2C9) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cytochrome P450 2C9 (CYP2C9) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP2C9 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cytochrome P450 2C9 (CYP2C9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cytochrome P450 2C9 (CYP2C9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cytochrome P450 2C9 (CYP2C9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cytochrome P450 2C9 (CYP2C9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CYP2C9 elisa kit

Tumor immunity

NCBI and Uniprot Product Information

• NCBI GI #: 13699818
• NCBI GeneID: 1559
• NCBI Accession #: NP_000762.2
• NCBI GenBank Nucleotide #: NM_000771.3
• Molecular Weight: 18,005 Da
• NCBI Official Full Name: cytochrome P450 2C9
• NCBI Official Synonym Full Names: cytochrome P450, family 2, subfamily C, polypeptide 9
• NCBI Official Symbol: CYP2C9
• NCBI Official Synonym Symbols: CPC9; CYP2C; CYP2C10; CYPIIC9; P450IIC9
• NCBI Protein Information: cytochrome P450 2C9
• UniProt Protein Name: Cytochrome P450 2C9
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2C9
• UniProt Synonym Gene Names: CYP2C10
• UniProt Entry Name: CP2C9_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and its expression is induced by rifampin. The enzyme is known to metabolize many xenobiotics, including phenytoin, tolbutamide, ibuprofen and S-warfarin. Studies identifying individuals who are poor metabolizers of phenytoin and tolbutamide suggest that this gene is polymorphic. The gene is located within a cluster of cytochrome P450 genes on chromosome 10q24. [provided by RefSeq, Jul 2008]

Uniprot Description

CYP2C9: Cytochromes P450 are a group of heme-thiolate monooxygenases. In liver microsomes, this enzyme is involved in an NADPH-dependent electron transport pathway. It oxidizes a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics. This enzyme contributes to the wide pharmacokinetics variability of the metabolism of drugs such as S- warfarin, diclofenac, phenytoin, tolbutamide and losartan. Belongs to the cytochrome P450 family.

Protein type: Secondary Metabolites Metabolism - limonene and pinene degradation; Xenobiotic Metabolism - metabolism by cytochrome P450; Cofactor and Vitamin Metabolism - retinol; Oxidoreductase; EC 1.14.14.1; Xenobiotic Metabolism - drug metabolism - cytochrome P450; Lipid Metabolism - linoleic acid; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 10q24

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; cytoplasm

Molecular Function: (S)-limonene 6-monooxygenase activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; (S)-limonene 7-monooxygenase activity; arachidonic acid epoxygenase activity; steroid hydroxylase activity; drug binding; oxidoreductase activity; oxygen binding; monooxygenase activity

Biological Process: steroid metabolic process; amide metabolic process; drug catabolic process; xenobiotic metabolic process; exogenous drug catabolic process; monoterpenoid metabolic process; arachidonic acid metabolic process; epoxygenase P450 pathway; drug metabolic process; monocarboxylic acid metabolic process; urea metabolic process

Disease: Coumarin Resistance