Mouse alpha-Synuclein Monoclonal Antibody | anti-Snca antibody

alpha-Synuclein Antibody

Cat. Num. AAA415137

• Gene Names: Snca; NACP; alphaSYN
• Applications: Immunofluorescence
• Purity: Affinity purified
• Synonyms: alpha-Synuclein; Monoclonal Antibody; alpha-Synuclein Antibody; anti-Snca antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Human and rodent
• Purity/Purification: Affinity purified
• Form/Format: Liquid
• Concentration: 100ug/100ul (varies by lot)
• Sequence Length: 140

• Applicable Applications for anti-Snca antibody: Immunofluorescence (IF)
• Application Notes: 1:500 for immunofluorescence.
• Immunogen: Alpha synuclein is a member of the synuclein family, the other two proteins being beta and gamma synuclein. Alpha synuclein was originally isolated as a major synaptic vesicle associated protein from the electric organ of the fish Torpedo (1). Direct homologues of alpha-synuclein are found in all vertebrates. Later work connected alpha-synuclein with human brain pathology, when a protein originally identified as a component of NAC, the "Non-Amyloid beta Component of Alzheimer's disease amyloid" proved to be a peptide derived from alpha-synuclein (2). The alpha-synuclein protein is therefore sometimes known as NAC precursor or NACP. Further work showed that alpha-synuclein is a major component of the Lewy bodies of Parkinson's disease and point mutations of alpha-synuclein proved to be causative of some forms of familial Parkinson's disease (3, 4, 5). Early onset Parkinson's disease may be caused by a duplication or triplication of one of the Alphasynuclein genes (6, 7). However, despite being discovered as as component of amyloid preparations, alpha-synuclein is apparently not a major component of the senile plaques of Alzheimer's disease (6). Early onset Parkinson's disease may be caused by a duplication or triplication of one of the alpha synuclein genes (7, 8). Alpha-synuclein is also found in the Lewy bodies of patients with diffuse Lewy body disease and inclusions in glial cells in the brains of patients with multiple system atrophy (MSA) and amyotrophic lateral sclerosis (ALS). Alpha-synuclein is heavily expressed in brain and appears to be localized primarily to presynaptic regions, though not with a typical synaptic vesicle distribution pattern. The synuclein proteins appear to have little 3D structure in solution, and probably belong to the family of "intrinsically unstructured proteins" which only adopt a well-defined conformation when bound to other proteins or membrane lipids (9). An excellent recent review of the role of alpha-synuclein in health and disease was recently published by Mark Cookson (10). The HGNC name for this protein is SNCA.
• Storage Buffer: PBS, pH 7.4 with 0.02% sodium azide.
• Preparation and Storage: This product is stable for several weeks at 4 degree C as an undiluted liquid. Dilute only prior to immediate use. For extended storage, aliquot contents and freeze at -20 degree C or below. Avoid cycles of freezing and thawing. Expiration date is one (1) year from date of receipt.

Western Blot (WB)

Testing Data #2

References

1. Maroteaux, L., J.T. Campanelli, and R.H. Scheller. Synuclein: a neuron-specific protein localized to the nucleus and presynaptic nerve terminal. J. Neurosci. 8:2804-2815 (1988).2. Lavedan, C. The Synuclein Family. Genome Research 8:871-880 (1998).3. Polymeropoulos, MH et al. Mutation in the alpha-synuclein gene identified in families with Parkinson's disease. Science 276: 2045-2047 (1997).4. Kruger, R et al. Ala30-to-Pro mutation in the gene encoding alpha-synuclein in Parkinson's disease. Nature Genet. 18: 106-108 (1998).5. Chartier-Harlin, M-C. et al. Alpha-synuclein locus duplication as a cause of familial Parkinson's disease. Lancet 364: 1167-1169 (2004).6. Singleton, AB.et al. Alpha-synuclein locus triplication causes Parkinson's disease. Science 302: 841 (2003).7. Culvenor, J.G. et al. Non-Abeta component of Alzheimer's disease amyloid (NAC) revisited: NAC and alpha-synuclein are not associated with Abeta amyloid. Am. J. Pathol. 155:1173-1181 (1999).8. Ibanez, P. et al. Causal relation between alpha-synuclein gene duplication and familial Parkinson's disease. Lancet 364:1169-1171 (2004).9. Dyson, HJ. and Wright, PE. Intrinsically unstructured proteins and their functions. Nature Reviews of Molecular and Cellular Biology 6:197-208 (2005).10. Cookson MR. Alpha-Synuclein and neuronal cell death. Mol Neurodeg 4:9 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 109638747
• NCBI GeneID: 20617
• NCBI Accession #: NP_001035916.1
• NCBI GenBank Nucleotide #: NM_001042451.1
• UniProt Accession #: O55042; Q3U130; Q9CXF8; Q9EQC3; Q9QUR3
• Molecular Weight: 14,485 Da
• NCBI Official Full Name: alpha-synuclein
• NCBI Official Synonym Full Names: synuclein, alpha
• NCBI Official Symbol: Snca
• NCBI Official Synonym Symbols: NACP; alphaSYN
• NCBI Protein Information: alpha-synuclein; alpha SYN; non-A4 component of amyloid; non-A beta component of AD amyloid
• UniProt Protein Name: Alpha-synuclein
• Protein Family: Alpha-synuclein
• UniProt Gene Name: Snca
• UniProt Synonym Gene Names: Syn; NACP
• UniProt Entry Name: SYUA_MOUSE

Uniprot Description

Function: May be involved in the regulation of dopamine release and transport.

Subunit structure: Interacts with UCHL1

By similarity.

Subcellular location: Cytoplasm

By similarity.

Post-translational modification: Phosphorylated, predominantly on serine residues. Phosphorylated on Tyr-125 upon osmotic stress

By similarity. Ref.9Ubiquitinated. The predominant conjugate is the diubiquitinated form

By similarity.Acetylation at Met-1 seems to be important for proper folding and native oligomeric structure

By similarity.

Sequence similarities: Belongs to the synuclein family.