Typical Testing Data/Standard Curve (for reference only)
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Human Cytochrome b 245 light chain (CYBA) ELISA Kit | CYBA elisa kit
Human Cytochrome b 245 light chain (CYBA) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
()
Typical Testing Data/Standard Curve (for reference only)
()
Principle of the Assay: CYBA ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-CYBA antibody and an CYBA-HRP conjugate. The assay sample and buffer are incubated together with CYBA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CYBA concentration since CYBA from samples and CYBA-HRP conjugate compete for the anti-CYBA antibody binding site. Since the number of sites is limited, as more sites are occupied by CYBA from the sample, fewer sites are left to bind CYBA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYBA concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Cytochrome b is comprised of a light chain (alpha) and a heavy chain (beta). This gene encodes the light, alpha subunit which has been proposed as a primary component of the microbicidal oxidase system of phagocytes. Mutations in this gene are associated with autosomal recessive chronic granulomatous disease (CGD), that is characterized by the failure of activated phagocytes to generate superoxide, which is important for the microbicidal activity of these cells. [provided by RefSeq, Jul 2008]
Uniprot Description
CYBA: Critical component of the membrane-bound oxidase of phagocytes that generates superoxide. Associates with NOX3 to form a functional NADPH oxidase constitutively generating superoxide. Defects in CYBA are a cause of chronic granulomatous disease autosomal recessive cytochrome-b-negative (ARCGD). Chronic granulomatous disease is a genetically heterogeneous disorder characterized by the inability of neutrophils and phagocytes to kill microbes that they have ingested. Patients suffer from life-threatening bacterial/fungal infections. Belongs to the p22phox family.
Protein type: Membrane protein, integral; EC 1.-.-.-; Oxidoreductase
Chromosomal Location of Human Ortholog: 16q24
Cellular Component: Golgi apparatus; phagocytic vesicle membrane; cell soma; membrane; mitochondrion; dendrite; apical plasma membrane; plasma membrane; endosome; NADPH oxidase complex; secretory granule
Molecular Function: protein binding; electron carrier activity; protein heterodimerization activity; metal ion binding; superoxide-generating NADPH oxidase activity; heme binding; SH3 domain binding
Biological Process: response to nutrient levels; response to drug; respiratory burst; interaction with host; angiotensin-mediated regulation of glomerular filtration; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; positive regulation of cell growth; regulation of release of sequestered calcium ion into cytosol; antigen processing and presentation of peptide antigen via MHC class I; cytochrome complex assembly; innate immune response; antigen processing and presentation of exogenous peptide antigen via MHC class I; positive regulation of endothelial cell proliferation; smooth muscle hypertrophy; inflammatory response; vascular endothelial growth factor receptor signaling pathway; superoxide release; hydrogen peroxide biosynthetic process
Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-negative
Research Articles on CYBA
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Product Notes
The Human CYBA cyba (Catalog #AAA7244670) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7244670 ELISA Kit recognizes Human CYBA. It is sometimes possible for the material contained within the vial of "Cytochrome b 245 light chain (CYBA), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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