Rabbit anti-Human, Mouse PLOD2 Polyclonal Antibody | anti-PLOD2 antibody

PLOD2 (Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2, Lysyl Hydroxylase 2, LH2) (PE)

Cat. Num. AAA6389844

• Gene Names: PLOD2; LH2; TLH; BRKS2
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: PLOD2; Polyclonal Antibody; PLOD2 (Procollagen-lysine; 2-oxoglutarate 5-dioxygenase 2; Lysyl Hydroxylase 2; LH2) (PE); anti-PLOD2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human PLOD2. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Sequence Length: 758

• Applicable Applications for anti-PLOD2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human PLOD2, aa1-758 (NP_891988.1).
• Immunogen Sequence: MGGCTVKPQLLLLALVLHPWNPCLGADSEKPSSIPTDKLLVITVATKESDGFHRFMQSAKYFNYTVKVLGQGEEWRGGDGINSIGGGQKVRLMKEVMEHYADQDDLVVMFTECFDVIFAGGPEEVLKKFQKANHKVVFAADGILWPDKRLADKYPVVHIGKRYLNSGGFIGYAPYVNRIVQQWNLQDNDDDQLFYTKVYIDPLKREAINITLDHKCKIFQTLNGAVDEVVLKFENGKARAKNTFYETLPVAINGNGPTKILLNYFGNYVPNSWTQDNGCTLCEFDTVDLSAVDVHPNVSIGVFIEQPTPFLPRFLDILLTLDYPKEALKLFIHNKEVYHEKDIKVFFDKAKHEIKTIKIVGPEENLSQAEARNMGMDFCRQDEKCDYYFSVDADVVLTNPRTLKILIEQNRKIIAPLVTRHGKLWSNFWGALSPDGYYARSEDYVDIVQGNRVGVWNVPYMANVYLIKGKTLRSEMNERNYFVRDKLDPDMALCRNAREMTLQREKDSPTPETFQMLSPPKGVFMYISNRHEFGRLLSTANYNTSHYNNDLWQIFENPVDWKEKYINRDYSKIFTENIVEQPCPDVFWFPIFSEKACDELVEEMEHYGKWSGGKHHDSRISGGYENVPTDDIHMKQVDLENVWLHFIREFIAPVTLKVFAGYYTKGFALLNFVVKYSPERQRSLRPHHDASTFTINIALNNVGEDFQGGGCKFLRYNCSIESPRKGWSFMHPGRLTHLHEGLPVKNGTRYIAVSFIDP
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(PLOD2 rabbit polyclonal antibody. Western Blot analysis of PLOD2 expression in HeLa.)

Western Blot (WB)

(Western Blot analysis of PLOD2 expression in transfected 293T cell line by PLOD2 polyclonal antibody. Lane 1: PLOD2 transfected lysate (87.1kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-PLOD2 antibody

Forms hydroxylysine residues in-Xaa-Lys-Gly-sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links.

Product Categories/Family for anti-PLOD2 antibody

Antibodies; Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 33636742
• NCBI GeneID: 5352
• NCBI Accession #: NP_891988.1
• NCBI GenBank Nucleotide #: NM_182943.2
• UniProt Accession #: O00469
• NCBI Official Full Name: procollagen-lysine,2-oxoglutarate 5-dioxygenase 2 isoform 1
• NCBI Official Synonym Full Names: procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
• NCBI Official Symbol: PLOD2
• NCBI Official Synonym Symbols: LH2; TLH; BRKS2
• NCBI Protein Information: procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
• UniProt Protein Name: Procollagen-lysine,2-oxoglutarate 5-dioxygenase 2
• Protein Family: Procollagen-lysine,2-oxoglutarate 5-dioxygenase
• UniProt Gene Name: PLOD2
• UniProt Synonym Gene Names: LH2
• UniProt Entry Name: PLOD2_HUMAN

NCBI Description

The protein encoded by this gene is a membrane-bound homodimeric enzyme that is localized to the cisternae of the rough endoplasmic reticulum. The enzyme (cofactors iron and ascorbate) catalyzes the hydroxylation of lysyl residues in collagen-like peptides. The resultant hydroxylysyl groups are attachment sites for carbohydrates in collagen and thus are critical for the stability of intermolecular crosslinks. Some patients with Ehlers-Danlos syndrome type VIB have deficiencies in lysyl hydroxylase activity. Mutations in the coding region of this gene are associated with Bruck syndrome. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

PLOD2: Forms hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. These hydroxylysines serve as sites of attachment for carbohydrate units and are essential for the stability of the intermolecular collagen cross-links. Defects in PLOD2 are the cause of Bruck syndrome type 2 (BRKS2). Bruck syndrome, also known as osteogenesis imperfecta with congenital joint contractures, is an autosomal recessive disease characterized by generalized osteopenia, joint contractures at birth, fragile bones and short stature. It can be distinguished from osteogenesis imperfecta by the absence of hearing loss and dentinogenesis imperfecta, and by the presence of clubfoot and congenital joint limitations. The molecular defect is an aberrant cross-linking of bone collagen, due to underhydroxylation of lysine residues within the telopeptides of type I collagen, whereas the lysine residues in the triple helix are normal. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Endoplasmic reticulum; Amino Acid Metabolism - lysine degradation; Oxidoreductase; EC 1.14.11.4

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: endoplasmic reticulum membrane; endoplasmic reticulum; rough endoplasmic reticulum membrane

Molecular Function: L-ascorbic acid binding; iron ion binding; procollagen-lysine 5-dioxygenase activity

Biological Process: extracellular matrix organization and biogenesis; response to hypoxia; protein modification process

Disease: Bruck Syndrome 2