Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cyclin-dependent kinase 16 (PCTK1) Standard Curve.)
Human Cyclin-Dependent Kinase 16 (PCTK1) ELISA Kit | PCTK1 elisa kit
Human Cyclin-Dependent Kinase 16 (PCTK1) ELISA Kit
Gene Names
CDK16; PCTK1; PCTAIRE; PCTAIRE1; PCTGAIRE
Reactivity
Human
Synonyms
Cyclin-Dependent Kinase 16 (PCTK1); Human Cyclin-Dependent Kinase 16 (PCTK1) ELISA Kit; PCTK1; FLJ16665; PCTAIRE; PCTAIRE1; PCTGAIRE; OTTHUMP00000023209; PCTAIRE protein kinase 1; PCTAIRE-motif protein kinase 1; serine/threonine-protein kinase; PCTK1 elisa kit
Reactivity
Human
Specificity
Human Cyclin-dependent kinase 16 (PCTK1) ELISA Kit has high sensitivity and excellent specificity for detection of Human PCTK1. No significant cross-reactivity or interference between Human PCTK1 and analogues was observed.
Sequence Length
496
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cyclin-dependent kinase 16 (PCTK1) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cyclin-dependent kinase 16 (PCTK1) Standard Curve.)
Related Product Information for PCTK1 elisa kit
Description: This Human Cyclin-dependent kinase 16 (PCTK1) ELISA Kit employs a two-site sandwich ELISA to quantitate PCTK1 in samples. An antibody specific for PCTK1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PCTK1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PCTK1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PCTK1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: CDK16 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells. This gene is thought to escape X inactivation. Two transcript variants encoding the same protein have been found for this gene. The 3 PCTAIRE kinases are 65% identical to one another (80% within the catalytic domain). PCTAIRE1 is ubiquitously expressed with the highest levels detected in the brain and testis. Human PCTAIRE cDNAs do not complement yeast cdc28 mutants and show other differences, suggesting that PCTAIRE kinases have different cellular functions from those described for CDC2.
Background: CDK16 belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells. This gene is thought to escape X inactivation. Two transcript variants encoding the same protein have been found for this gene. The 3 PCTAIRE kinases are 65% identical to one another (80% within the catalytic domain). PCTAIRE1 is ubiquitously expressed with the highest levels detected in the brain and testis. Human PCTAIRE cDNAs do not complement yeast cdc28 mutants and show other differences, suggesting that PCTAIRE kinases have different cellular functions from those described for CDC2.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cyclin-dependent kinase 16 isoform 1
NCBI Official Synonym Full Names
cyclin dependent kinase 16
NCBI Official Symbol
CDK16
NCBI Official Synonym Symbols
PCTK1; PCTAIRE; PCTAIRE1; PCTGAIRE
NCBI Protein Information
cyclin-dependent kinase 16
UniProt Protein Name
Cyclin-dependent kinase 16
UniProt Gene Name
CDK16
UniProt Synonym Gene Names
PCTAIRE1; PCTK1
NCBI Description
The protein encoded by this gene belongs to the cdc2/cdkx subfamily of the ser/thr family of protein kinases. It may play a role in signal transduction cascades in terminally differentiated cells; in exocytosis; and in transport of secretory cargo from the endoplasmic reticulum. This gene is thought to escape X inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Dec 2009]
Uniprot Description
Protein kinase that plays a role in vesicle-mediated transport processes and exocytosis. Regulates GH1 release by brain neurons. Phosphorylates NSF, and thereby regulates NSF oligomerization. Required for normal spermatogenesis. Regulates neuron differentiation and dendrite development (). Plays a role in the regulation of insulin secretion in response to changes in blood glucose levels. Can phosphorylate CCNY at 'Ser-336' (in vitro).
Research Articles on PCTK1
1. CDK16 negatively modulates p53 signaling pathway to promote radioresistance in lung cancer cells.
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Product Notes
The Human PCTK1 cdk16 (Catalog #AAA9714948) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9714948 ELISA Kit recognizes Human PCTK1. It is sometimes possible for the material contained within the vial of "Cyclin-Dependent Kinase 16 (PCTK1), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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