Typical Testing Data/Standard Curve (for reference only)
(Human CXCL16 PicoKine ELISA Kit standard curve )
Human CXCL16 ELISA Kit | CXCL16 elisa kit
Human CXCL16 PicoKine ELISA Kit
Gene Names
CXCL16; SRPSOX; CXCLG16; SR-PSOX
Reactivity
Human
Synonyms
CXCL16; Human CXCL16 PicoKine ELISA Kit; C-X-C motif chemokine 16; chemokine (C-X-C motif) ligand 16; 0910001K24Rik; AV290116; BB024863; C X C motif chemokine 16; Chemokine (C X C motif) ligand 16; CXC chemokine ligand 16; CXCLG16; DN 491F10.6; Scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SCYB16; Small inducible cytokine B; Small inducible cytokine B16; Small inducible cytokine B16 precursor; SR PSOX; SR PSOX/CXCL16 scavenger receptor for phosphatidylserine and oxidized low density lipoprotein; SRPSOX; Transmembrane chemokine CXCL16; Zinc finger; MYND-type containing 15; Zmynd15; CXCL16 elisa kit
Reactivity
Human
Specificity
Natural and recombinant human CXCL16
Sequence Length
254
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
93.7pg/ml-6000pg/ml
Sensitivity
< 10pg/ml
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Human CXCL16 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Human CXCL16 PicoKine ELISA Kit standard curve )
Related Product Information for CXCL16 elisa kit
Principle of the assay: human CXCL16 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CXCL16 has been precoated onto 96-well plates. Standards(E.coli,N49-P137) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CXCL16 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CXCL16 amount of sample captured in plate.
Background: Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells.[1] CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16 (CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.
Background: Chemokine (C-X-C motif) ligand 16 (CXCL16) is a small cytokine belonging to the CXC chemokine family. Larger than other chemokines (with 254 amino acids), CXCL16 is composed of a CXC chemokine domain, a mucin-like stalk, a transmembrane domain and a cytoplasmic tail containing a potential tyrosine phosphorylation site that may bind SH2. These are unusual features for a chemokine, and allow CXCL16 to be expressed as a cell surface bound molecule, as well as a soluble chemokine. CXCL16 is produced by dendritic cells found in the T cell zones of lymphoid organs, and by cells found in the red pulp of the spleen. Cells that bind and migrate in response to CXCL16 include several subsets of T cells, and natural killer T (NKT) cells.[1] CXCL16 interacts with the chemokine receptorCXCR6, also known as Bonzo. The novel CXC-chemokine ligand 16 (CXCL16) functions as transmembrane adhesion molecule on the surface of APCs and as a soluble chemoattractant for activated T cells. Expression of CXCL16 is induced by the inflammatory cytokines IFN-gamma and TNF-alpha. The gene for human CXCL16 is located on chromosome 17.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
27,579 Da
NCBI Official Full Name
C-X-C motif chemokine 16
NCBI Official Synonym Full Names
chemokine (C-X-C motif) ligand 16
NCBI Official Symbol
CXCL16
NCBI Official Synonym Symbols
SRPSOX; CXCLG16; SR-PSOX
NCBI Protein Information
C-X-C motif chemokine 16; CXC chemokine ligand 16; small-inducible cytokine B16; transmembrane chemokine CXCL16; scavenger receptor for phosphatidylserine and oxidized low density lipoprotein
UniProt Protein Name
C-X-C motif chemokine 16
Protein Family
UniProt Gene Name
CXCL16
UniProt Synonym Gene Names
SCYB16; SRPSOX; SR-PSOX
UniProt Entry Name
CXL16_HUMAN
Uniprot Description
CXCL16: Acts as a scavenger receptor on macrophages, which specifically binds to OxLDL (oxidized low density lipoprotein), suggesting that it may be involved in pathophysiology such as atherogenesis. Induces a strong chemotactic response. Induces calcium mobilization. Binds to CXCR6/Bonzo. Belongs to the intercrine alpha (chemokine CxC) family.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 17p13
Cellular Component: extracellular space; membrane; integral to membrane; plasma membrane; extracellular region
Molecular Function: low-density lipoprotein receptor activity; chemokine activity; scavenger receptor activity
Biological Process: receptor-mediated endocytosis; response to cytokine stimulus; chemotaxis; positive regulation of cell growth; positive regulation of cell migration
Research Articles on CXCL16
Similar Products
Product Notes
The Human CXCL16 cxcl16 (Catalog #AAA176487) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA176487 ELISA Kit recognizes Human CXCL16. It is sometimes possible for the material contained within the vial of "CXCL16, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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