Human CREB Regulated Transcription Coactivator 3 (CRTC3) ELISA Kit | CRTC3 elisa kit

Human CREB Regulated Transcription Coactivator 3 (CRTC3) ELISA Kit

Cat. Num. AAA451422

• Gene Names: CRTC3; TORC3; TORC-3
• Reactivity: Human
• Synonyms: CREB Regulated Transcription Coactivator 3 (CRTC3); Human CREB Regulated Transcription Coactivator 3 (CRTC3) ELISA Kit; TORC3; Transducer of regulated cAMP response element-binding protein 3; CRTC3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CRTC3. No significant cross-reactivity or interference between CRTC3 and analogues was observed.
• Sequence Length: 618

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.112ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CRTC3 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CRTC3 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CRTC3 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CRTC3 in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CRTC3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CRTC3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CRTC3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CRTC3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 110431352
• NCBI GeneID: 64784
• NCBI Accession #: NP_001036039.1
• NCBI GenBank Nucleotide #: NM_001042574.2
• UniProt Accession #: Q6UUV7; Q6DK61; Q6DK62; Q8NF38; Q9H6U2
• Molecular Weight: 66,830 Da
• NCBI Official Full Name: CREB-regulated transcription coactivator 3 isoform b
• NCBI Official Synonym Full Names: CREB regulated transcription coactivator 3
• NCBI Official Symbol: CRTC3
• NCBI Official Synonym Symbols: TORC3; TORC-3
• NCBI Protein Information: CREB-regulated transcription coactivator 3
• UniProt Protein Name: CREB-regulated transcription coactivator 3
• Protein Family: CREB-regulated transcription coactivator
• UniProt Gene Name: CRTC3
• UniProt Synonym Gene Names: TORC3; TORC-3; Transducer of CREB protein 3

NCBI Description

This gene is a member of the CREB regulated transcription coactivator gene family. This family regulates CREB-dependent gene transcription in a phosphorylation-independent manner and may be selective for cAMP-responsive genes. The protein encoded by this gene may induce mitochondrial biogenesis and attenuate catecholamine signaling in adipose tissue. A translocation event between this gene and Notch coactivator mastermind-like gene 2, which results in a fusion protein, has been reported in mucoepidermoid carcinomas. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

Transcriptional coactivator for CREB1 which activates transcription through both consensus and variant cAMP response element (CRE) sites. Acts as a coactivator, in the SIK/TORC signaling pathway, being active when dephosphorylated and acts independently of CREB1 'Ser-133' phosphorylation. Enhances the interaction of CREB1 with TAF4. Regulates the expression of specific CREB-activated genes such as the steroidogenic gene, StAR. Potent coactivator of PPARGC1A and inducer of mitochondrial biogenesis in muscle cells. Also coactivator for TAX activation of the human T-cell leukemia virus type 1 (HTLV-1) long terminal repeats (LTR).