Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Creatine kinase U-type, mitochondrial (CKMT1A) Standard Curve.)
Human Creatine Kinase U-Type, Mitochondrial (CKMT1A) ELISA Kit | CKMT1A elisa kit
Human Creatine Kinase U-Type, Mitochondrial (CKMT1A) ELISA Kit
Reactivity
Human
Synonyms
Creatine Kinase U-Type; Mitochondrial (CKMT1A); Human Creatine Kinase U-Type; Mitochondrial (CKMT1A) ELISA Kit; CKMT1A; CKMT1; FLJ50967; UMTCK; acidic-type mitochondrial creatine kinase; creatine kinase; mitochondrial 1 (ubiquitous); CKMT1A elisa kit
Reactivity
Human
Specificity
Human Creatine kinase U-type, mitochondrial (CKMT1A) ELISA Kit has high sensitivity and excellent specificity for detection of Human CKMT1A. No significant cross-reactivity or interference between Human CKMT1A and analogues was observed.
Sequence Length
417
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Creatine kinase U-type, mitochondrial (CKMT1A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Creatine kinase U-type, mitochondrial (CKMT1A) Standard Curve.)
Related Product Information for CKMT1A elisa kit
Description: This Human Creatine kinase U-type, mitochondrial (CKMT1A) ELISA Kit employs a two-site sandwich ELISA to quantitate CKMT1A in samples. An antibody specific for CKMT1A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any CKMT1A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for CKMT1A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of CKMT1A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Creatine kinase, mitochondrial 1A also known as CKMT1A is one of two genes which encode the ubiquitous mitochondrial creatine kinase (ubiquitous mtCK or CKMT1). Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase.
Background: Creatine kinase, mitochondrial 1A also known as CKMT1A is one of two genes which encode the ubiquitous mitochondrial creatine kinase (ubiquitous mtCK or CKMT1). Mitochondrial creatine (MtCK) kinase is responsible for the transfer of high energy phosphate from mitochondria to the cytosolic carrier, creatine. It belongs to the creatine kinase isoenzyme family. It exists as two isoenzymes, sarcomeric MtCK (CKMT2) and ubiquitous MtCK, encoded by separate genes. Mitochondrial creatine kinase occurs in two different oligomeric forms: dimers and octamers, in contrast to the exclusively dimeric cytosolic creatine kinase isoenzymes. Ubiquitous mitochondrial creatine kinase has 80% homology with the coding exons of sarcomeric mitochondrial creatine kinase.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
creatine kinase U-type, mitochondrial isoform 1
UniProt Protein Name
Creatine kinase U-type, mitochondrial
UniProt Gene Name
CKMT1A
UniProt Synonym Gene Names
CKMT; Mia-CK; U-MtCK
UniProt Entry Name
KCRU_HUMAN
Uniprot Description
CKMT1A: Reversibly catalyzes the transfer of phosphate between ATP and various phosphogens (e.g. creatine phosphate). Creatine kinase isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Belongs to the ATP:guanido phosphotransferase family. Exists as an octamer composed of four MTCK homodimers. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Mitochondrial; Kinase, other; EC 2.7.3.2; Amino Acid Metabolism - arginine and proline
Chromosomal Location of Human Ortholog: 15q15
Cellular Component: mitochondrion; mitochondrial inner membrane
Molecular Function: creatine kinase activity; ATP binding
Biological Process: phosphorylation; creatine metabolic process
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Product Notes
The Human CKMT1A ckmt1a (Catalog #AAA9715943) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9715943 ELISA Kit recognizes Human CKMT1A. It is sometimes possible for the material contained within the vial of "Creatine Kinase U-Type, Mitochondrial (CKMT1A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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