Human Complement Factor I (CFI) ELISA Kit | CFI elisa kit

Human Complement Factor I (CFI) ELISA Kit

Cat. Num. AAA452799

• Gene Names: CFI; FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA
• Reactivity: Human
• Synonyms: Complement Factor I (CFI); Human Complement Factor I (CFI) ELISA Kit; C3B/C4B inactivator; Complement factor I; CFI elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CFI. No significant cross-reactivity or interference between CFI and analogues was observed.
• Sequence Length: 583

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/mL
• Sensitivity: 0.051ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CFI were tested 20 times on one plate, respectively.Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CFI were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CFI elisa kit

Intended Uses: This kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CFI in human serum, plasma and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CFI. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CFI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CFI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CFI in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119392081
• NCBI GeneID: 3426
• NCBI Accession #: NP_000195.2
• NCBI GenBank Nucleotide #: NM_000204.4
• UniProt Accession #: P05156; O60442
• Molecular Weight: 65,750 Da
• NCBI Official Full Name: complement factor I isoform 2 preproprotein
• NCBI Official Synonym Full Names: complement factor I
• NCBI Official Symbol: CFI
• NCBI Official Synonym Symbols: FI; IF; KAF; AHUS3; ARMD13; C3BINA; C3b-INA
• NCBI Protein Information: complement factor I
• UniProt Protein Name: Complement factor I
• Protein Family: Complement factor
• UniProt Gene Name: CFI
• UniProt Synonym Gene Names: IF

NCBI Description

This gene encodes a serine proteinase that is essential for regulating the complement cascade. The encoded preproprotein is cleaved to produce both heavy and light chains, which are linked by disulfide bonds to form a heterodimeric glycoprotein. This heterodimer can cleave and inactivate the complement components C4b and C3b, and it prevents the assembly of the C3 and C5 convertase enzymes. Defects in this gene cause complement factor I deficiency, an autosomal recessive disease associated with a susceptibility to pyogenic infections. Mutations in this gene have been associated with a predisposition to atypical hemolytic uremic syndrome, a disease characterized by acute renal failure, microangiopathic hemolytic anemia and thrombocytopenia. Primary glomerulonephritis with immune deposits and age-related macular degeneration are other conditions associated with mutations of this gene. [provided by RefSeq, Dec 2015]

Uniprot Description

Responsible for cleaving the alpha-chains of C4b and C3b in the presence of the cofactors C4-binding protein and factor H respectively.