Rat CDGSH Iron Sulfur Domain Protein 1 (CISD1) ELISA Kit | CISD1 elisa kit

Rat CDGSH Iron Sulfur Domain Protein 1 (CISD1) ELISA Kit

Cat. Num. AAA2706889

• Gene Names: CISD1; ZCD1; MDS029; C10orf70; mitoNEET
• Reactivity: Rat
• Synonyms: CDGSH Iron Sulfur Domain Protein 1 (CISD1); Rat CDGSH Iron Sulfur Domain Protein 1 (CISD1) ELISA Kit; ZCD1; mitoNEET; C10orf70; Zinc Finger; CDGSH-Type Domain 1; CISD1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CDGSH Iron Sulfur Domain Protein 1 (CISD1).; No significant cross-reactivity or interference between CDGSH Iron Sulfur Domain Protein 1 (CISD1) and analogues was observed.
• Sequence Length: 108

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.054ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CDGSH Iron Sulfur Domain Protein 1 (CISD1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CDGSH Iron Sulfur Domain Protein 1 (CISD1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CISD1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CDGSH Iron Sulfur Domain Protein 1 (CISD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CDGSH Iron Sulfur Domain Protein 1 (CISD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CDGSH Iron Sulfur Domain Protein 1 (CISD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of CDGSH Iron Sulfur Domain Protein 1 (CISD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 37590612
• NCBI GeneID: 55847
• Molecular Weight: 12,199 Da
• NCBI Official Full Name: CDGSH iron sulfur domain 1
• NCBI Official Synonym Full Names: CDGSH iron sulfur domain 1
• NCBI Official Symbol: CISD1
• NCBI Official Synonym Symbols: ZCD1; MDS029; C10orf70; mitoNEET
• NCBI Protein Information: CDGSH iron-sulfur domain-containing protein 1
• UniProt Protein Name: CDGSH iron-sulfur domain-containing protein 1
• Protein Family: CDGSH iron-sulfur domain-containing protein
• UniProt Gene Name: CISD1
• UniProt Synonym Gene Names: C10orf70; ZCD1

NCBI Description

This gene encodes a protein with a CDGSH iron-sulfur domain and has been shown to bind a redox-active [2Fe-2S] cluster. The encoded protein has been localized to the outer membrane of mitochondria and is thought to play a role in regulation of oxidation. Genes encoding similar proteins are located on chromosomes 4 and 17, and a pseudogene of this gene is located on chromosome 2. [provided by RefSeq, Feb 2012]

Uniprot Description

Plays a key role in regulating maximal capacity for electron transport and oxidative phosphorylation (). May be involved in Fe-S cluster shuttling and/or in redox reactions.