Rabbit anti-Human LEO1 Polyclonal Antibody | anti-LEO1 antibody

Rabbit Polyclonal to Human LEO1

Cat. Num. AAA247998

• Gene Names: LEO1; RDL
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Immunoaffinity Purified
• Synonyms: LEO1; Polyclonal Antibody; Rabbit Polyclonal to Human LEO1; Anti-LEO1 Antibody (N-Terminus) IHC-plus; RDL; Human LEO1; anti-LEO1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS with 0.09% (w/v) sodium azide
• Sequence Length: 666

• Applicable Applications for anti-LEO1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB)
• Application Notes: IHC-P (10 ug/ml), WB (1:1000)
• Target Species: Human
• Immunogen Description: KLH conjugated synthetic peptide selected from the N-terminal region of human LEO1.
• Immunogen Type: Synthetic peptide
• Immunogen: LEO1 antibody was raised against kLH conjugated synthetic peptide selected from the N-terminal region of human LEO1.
• Antigen Modification: N-Terminus
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-LEO1 antibody IHC staining of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Western Blot (WB)

(Western blot of anti-Leo1 Antibody (N-term) in HL60 cell line lysates (35 ug/lane). Leo1(arrow) was detected using the purified Pab (1:150 dilution).)

Western Blot (WB)

(Western blot of Leo1 (arrow) using rabbit polyclonal Leo1 Antibody (Human N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the Leo1 gene (Lane 2).)

NCBI and Uniprot Product Information

• NCBI GI #: 20270337
• NCBI GeneID: 123169
• NCBI Accession #: NP_620147.1
• NCBI GenBank Nucleotide #: NM_138792.3
• UniProt Accession #: Q8WVC0; Q96N99
• Molecular Weight: 68,004 Da
• NCBI Official Full Name: RNA polymerase-associated protein LEO1 isoform 1
• NCBI Official Synonym Full Names: Leo1, Paf1/RNA polymerase II complex component, homolog (S. cerevisiae)
• NCBI Official Symbol: LEO1
• NCBI Official Synonym Symbols: RDL
• NCBI Protein Information: RNA polymerase-associated protein LEO1; replicative senescence down-regulated leo1-like protein
• UniProt Protein Name: RNA polymerase-associated protein LEO1
• Protein Family: RNA polymerase-associated protein
• UniProt Gene Name: LEO1
• UniProt Synonym Gene Names: RDL
• UniProt Entry Name: LEO1_HUMAN

NCBI Description

LEO1, parafibromin (CDC73; MIM 607393), CTR9 (MIM 609366), and PAF1 (MIM 610506) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]).[supplied by OMIM, Mar 2008]

Uniprot Description

LEO1: a component of the PAF1 multifunctional complex involved in initiation and elongation, histone methylation and RNA processing. The PAF1 complex interacts with POLR2A. Overexpression of LEO1 induces cell growth arrest and premature senescence of fibroblasts. The PAF1 complex consists of at least of CDC73, PAF1, LEO1 and CTR9. Interacts with CDC73. Two isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: nucleoplasm; nucleolus

Molecular Function: protein binding

Biological Process: histone monoubiquitination; Wnt receptor signaling pathway; negative regulation of myeloid cell differentiation; transcription, DNA-dependent; endodermal cell fate commitment; positive regulation of RNA elongation from RNA polymerase II promoter; histone H2B ubiquitination; stem cell maintenance; positive regulation of mRNA 3'-end processing; mRNA polyadenylation; positive regulation of transcription from RNA polymerase II promoter