Human Collectin ELISA Kit | COLEC11 elisa kit

Human Collectin ELISA Kit

Cat. Num. AAA267119

• Gene Names: COLEC11; 3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb
• Reactivity: Human
• Synonyms: Collectin; Human Collectin ELISA Kit; COLEC11 elisa kit

• Reactivity: Human
• Specificity: No cross-reaction with other factors.
• Sequence Length: 197

• Samples: human serum, plasma or cell culture supernatant
• Detection Range: 2000 pg/ml-31.2 pg/ml
• Sensitivity: 12 pg/mL
• Intra-assay Precision: ? 8%
• Inter-assay Precision: ? 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for COLEC11 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is human COLEC monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 156765940
• NCBI GeneID: 78989
• Molecular Weight: 30,200 Da
• NCBI Official Full Name: collectin kidney K1
• NCBI Official Synonym Full Names: collectin sub-family member 11
• NCBI Official Symbol: COLEC11
• NCBI Official Synonym Symbols: 3MC2; CLK1; CL-K1-I; CL-K1-II; CL-K1-IIa; CL-K1-IIb
• NCBI Protein Information: collectin-11; Collectin K1; collectin kidney protein 1
• UniProt Protein Name: Collectin-11
• Protein Family: Collectin
• UniProt Gene Name: COLEC11
• UniProt Synonym Gene Names: UNQ596/PRO1182; CL-K1
• UniProt Entry Name: COL11_HUMAN

NCBI Description

This gene encodes a member of the collectin family of C-type lectins that possess collagen-like sequences and carbohydrate recognition domains. Collectins are secreted proteins that play important roles in the innate immune system by binding to carbohydrate antigens on microorganisms, facilitating their recognition and removal. The encoded protein binds to multiple sugars with a preference for fucose and mannose. Mutations in this gene are a cause of 3MC syndrome-2. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, Dec 2011]

Uniprot Description

COLEC11: Lectin that binds to various sugars including fucose and mannose. Has a higher affinity for fucose compared to mannose. Does not bind to glucose, N-acetylglucosamine and N- acetylgalactosamine. Also binds lipopolysaccharides (LPS). Involved in fundamental development serving as a guidance cue for neural crest cell migration. Defects in COLEC11 are the cause of 3MC syndrome type 2 (3MC2). 3MC2 is an a disorder characterized by facial dysmorphism that includes hypertelorism, blepharophimosis, blepharoptosis and highly archedDE eyebrows, cleft lip and/or palate, craniosynostosis, learning disability and genital, limb and vesicorenal anomalies. The term 3MC syndrome includes Carnevale, Mingarelli, Malpuech, and Michels syndromes. Belongs to the COLEC10/COLEC11 family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 2p25.3

Cellular Component: collagen; extracellular region

Molecular Function: mannose binding

Biological Process: receptor-mediated endocytosis; multicellular organismal development; developmental process

Disease: 3mc Syndrome 2