Human Coiled Coil And C2 Domain Containing Protein 1A (CC2D1A) ELISA Kit | CC2D1A elisa kit

Human Coiled Coil And C2 Domain Containing Protein 1A (CC2D1A) ELISA Kit

Cat. Num. AAA454374

• Gene Names: CC2D1A; MRT3; FREUD-1; Freud-1/Aki1
• Reactivity: Human
• Synonyms: Coiled Coil And C2 Domain Containing Protein 1A (CC2D1A); Human Coiled Coil And C2 Domain Containing Protein 1A (CC2D1A) ELISA Kit; CC2D1A elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CC2D1A. No significant cross-reactivity or interference between CC2D1A and analogues was observed.
• Sequence Length: 951

• Samples: Tissue homogenates and other biological fluids.
• Assay Type: Sandwich
• Detection Range: 0.312-20ng/mL
• Sensitivity: 0.128ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CC2D1A were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CC2D1A were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CC2D1A elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of CC2D1A in human tissue homogenates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CC2D1A. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to CC2D1A. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CC2D1A, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CC2D1A in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 96974985
• NCBI GeneID: 54862
• NCBI Accession #: NP_060191.3
• NCBI GenBank Nucleotide #: NM_017721.4
• UniProt Accession #: Q6P1N0; Q7Z435; Q86XV0; Q8NF89; Q9H603; Q9NXI1
• Molecular Weight: 103,934 Da
• NCBI Official Full Name: coiled-coil and C2 domain-containing protein 1A
• NCBI Official Synonym Full Names: coiled-coil and C2 domain containing 1A
• NCBI Official Symbol: CC2D1A
• NCBI Official Synonym Symbols: MRT3; FREUD-1; Freud-1/Aki1
• NCBI Protein Information: coiled-coil and C2 domain-containing protein 1A
• UniProt Protein Name: Coiled-coil and C2 domain-containing protein 1A
• Protein Family: Coiled-coil and C2 domain-containing protein
• UniProt Gene Name: CC2D1A
• UniProt Synonym Gene Names: AKI1; FRE under dual repression-binding protein 1; Freud-1

NCBI Description

This gene encodes a transcriptional repressor that binds to a conserved 14-bp 5'-repressor element and regulates expression of the 5-hydroxytryptamine (serotonin) receptor 1A gene in neuronal cells. The DNA binding and transcriptional repressor activities of the protein are inhibited by calcium. A mutation in this gene results in nonsyndromic mental retardation-3.[provided by RefSeq, Oct 2009]

Uniprot Description

Transcription factor that binds specifically to the DRE (dual repressor element) and represses HTR1A gene transcription in neuronal cells. The combination of calcium and ATP specifically inactivates the binding with FRE. May play a role in the altered regulation of HTR1A associated with anxiety and major depression. Mediates HDAC-independent repression of HTR1A promoter in neuronal cell. Performs essential function in controlling functional maturation of synapses (). Plays distinct roles depending on its localization. When cytoplasmic, acts as a scaffold protein in the PI3K/PDK1/AKT pathway. Repressor of HTR1A when nuclear. In the centrosome, regulates spindle pole localization of the cohesin subunit SCC1/RAD21, thereby mediating centriole cohesion during mitosis.