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Typical Testing Data/Standard Curve (for reference only)

Human Coagulation factor V (F5) ELISA Kit | F5 elisa kit

Human Coagulation factor V (F5) ELISA Kit

Gene Names
F5; FVL; PCCF; THPH2; RPRGL1
Reactivity
Human
Synonyms
Coagulation factor V (F5); Human Coagulation factor V (F5) ELISA Kit; F5 elisa kit
Ordering
For Research Use Only!
Reactivity
Human
Sequence Length
2224
Samples
Serum, Plasma, Cell Culture Supernates, Cell Lysates, Tissue Homogenates
Assay Type
Quantitative Sandwich
Detection Range
20U/ml-4000U/ml
Sensitivity
9.33U/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision. Intra-Assay: CV<8%
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision. CV(%) = SD/mean x 100. Inter-Assay: CV<10%
Preparation and Storage
Store at 2 to 8 degree C for 6 months.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for F5 elisa kit
Intended Uses: This sandwich kit is for the accurate quantitative detection of human Cluster of Differentiation 8 (also known as CD8) in serum, plasma, cell culture supernates, cell lysates, tissue homogenates.

Principle of the Assay: This kit is an Enzyme-Linked Immunosorbent Assay (ELISA). The plate has been pre-coated with human CD8 antibody. CD8 present in the sample is added and binds to antibodies coated on the wells. And then biotinylated human CD8 Antibody is added and binds to CD8 in the sample. Then Streptavidin-HRP is added and binds to the Biotinylated CD8 antibody. After incubation unbound Streptavidin-HRP is washed away during a washing step. Substrate solution is then added and color develops in proportion to the amount of human CD8. The reaction is terminated by addition of acidic stop solution and absorbance is measured at 450 nm.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
251,703 Da
NCBI Official Full Name
coagulation factor V preproprotein
NCBI Official Synonym Full Names
coagulation factor V
NCBI Official Symbol
F5
NCBI Official Synonym Symbols
FVL; PCCF; THPH2; RPRGL1
NCBI Protein Information
coagulation factor V
UniProt Protein Name
Coagulation factor V
Protein Family
UniProt Gene Name
F5

NCBI Description

This gene encodes an essential cofactor of the blood coagulation cascade. This factor circulates in plasma, and is converted to the active form by the release of the activation peptide by thrombin during coagulation. This generates a heavy chain and a light chain which are held together by calcium ions. The activated protein is a cofactor that participates with activated coagulation factor X to activate prothrombin to thrombin. Defects in this gene result in either an autosomal recessive hemorrhagic diathesis or an autosomal dominant form of thrombophilia, which is known as activated protein C resistance. [provided by RefSeq, Oct 2008]

Uniprot Description

Central regulator of hemostasis. It serves as a critical cofactor for the prothrombinase activity of factor Xa that results in the activation of prothrombin to thrombin.

Research Articles on F5

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Product Notes

The Human F5 f5 (Catalog #AAA167452) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA167452 ELISA Kit recognizes Human F5. It is sometimes possible for the material contained within the vial of "Coagulation factor V (F5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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