Typical Testing Data/Standard Curve (for reference only)
Human Claudin 5 (CLDN5) ELISA Kit | CLDN5 elisa kit
Human Claudin 5 (CLDN5) ELISA Kit
Gene Names
CLDN5; AWAL; BEC1; TMVCF; CPETRL1
Reactivity
Human
Synonyms
Claudin 5 (CLDN5); Human Claudin 5 (CLDN5) ELISA Kit; AWAL; BEC1; CPETRL1; TMVCF; Transmembrane Protein Deleted In Velocardiofacial Syndrome; Transmembrane protein deleted in VCFS; CLDN5 elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of Claudin 5 (CLDN5).
No significant cross-reactivity or interference between Claudin 5 (CLDN5) and analogues was observed.
No significant cross-reactivity or interference between Claudin 5 (CLDN5) and analogues was observed.
Sequence Length
218
Assay Type
Double-antibody Sandwich
Samples
Serum, Plasma, Tissue homogenates, Cell lysates and Other Biological Fluids
Detection Range
31.2-2000pg/mL
Sensitivity
< 13.1pg/mL
Application
Enzyme-linked immunosorbent assay for Antigen Detection.
Intra-assay Precision (Precision within an assay)
3 samples with low, middle and high level Claudin 5 (CLDN5) were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays)
3 samples with low, middle and high level Claudin 5 (CLDN5) were tested on 3 different plates, 8 replicates in each plate.
CV(%) =
SD/meanX100
Intra-Assay
CV<10%
Inter-Assay
CV<12%
Preparation and Storage
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CLDN5 elisa kit
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 5 (CLDN5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 5 (CLDN5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 5 (CLDN5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Claudin 5 (CLDN5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Product Categories/Family for CLDN5 elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
23,147 Da
NCBI Official Full Name
claudin-5
NCBI Official Synonym Full Names
claudin 5
NCBI Official Symbol
CLDN5
NCBI Official Synonym Symbols
AWAL; BEC1; TMVCF; CPETRL1
NCBI Protein Information
claudin-5
UniProt Protein Name
Claudin-5
Protein Family
UniProt Gene Name
CLDN5
UniProt Synonym Gene Names
AWAL; TMVCF; TMDVCF
UniProt Entry Name
CLD5_HUMAN
NCBI Description
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Aug 2008]
Uniprot Description
Claudin-5: Plays a major role in tight junction-specific obliteration of the intercellular space. Belongs to the claudin family.
Protein type: Membrane protein, multi-pass; Cytoskeletal; Membrane protein, integral
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: tight junction; plasma membrane; integral to membrane; intercellular junction
Molecular Function: identical protein binding; structural molecule activity
Biological Process: calcium-independent cell-cell adhesion
Research Articles on CLDN5
Similar Products
Product Notes
The Human CLDN5 cldn5 (Catalog #AAA2024302) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2024302 ELISA Kit recognizes Human CLDN5. It is sometimes possible for the material contained within the vial of "Claudin 5 (CLDN5), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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