Human Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7) ELISA Kit | CHRNa7 elisa kit

Human Cholinergic Receptor, Nicotinic, Alpha 7 (CHRNa7) ELISA Kit

Cat. Num. AAA454030

• Gene Names: CHRNA7; NACHRA7; CHRNA7-2
• Reactivity: Human
• Synonyms: Cholinergic Receptor; Nicotinic; Alpha 7 (CHRNa7); Human Cholinergic Receptor; Alpha 7 (CHRNa7) ELISA Kit; ACBP; Anaphylatoxin inactivator; Arginine carboxypeptidase; Carboxypeptidase N catalytic chain; Carboxypeptidase N polypeptide 1; Carboxypeptidase N small subunit; CPN; CHRNa7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CHRNa7. No significant cross-reactivity or interference between CHRNa7 and analogues was observed.
• Sequence Length: 502

• Samples: human tissue homogenates, cell lysates or other biological fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: <0.112ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level CHRNa7 were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level CHRNa7 were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRNa7 elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of CHRNa7 in human tissue homogenates, cell lysates or other biological fluids.

NCBI and Uniprot Product Information

• NCBI GI #: 4502831
• NCBI GeneID: 1139
• NCBI Accession #: NP_000737.1
• NCBI GenBank Nucleotide #: NM_000746.5
• UniProt Accession #: P36544; Q15826; Q8IUZ4; Q96RH2; Q99555; Q9BXH0; A8K7Q4; B4DFS0
• Molecular Weight: 11,485 Da
• NCBI Official Full Name: neuronal acetylcholine receptor subunit alpha-7 isoform 1
• NCBI Official Synonym Full Names: cholinergic receptor nicotinic alpha 7 subunit
• NCBI Official Symbol: CHRNA7
• NCBI Official Synonym Symbols: NACHRA7; CHRNA7-2
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-7
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-7
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA7
• UniProt Synonym Gene Names: NACHRA7

NCBI Description

The nicotinic acetylcholine receptors (nAChRs) are members of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. The nAChRs are thought to be hetero-pentamers composed of homologous subunits. The proposed structure for each subunit is a conserved N-terminal extracellular domain followed by three conserved transmembrane domains, a variable cytoplasmic loop, a fourth conserved transmembrane domain, and a short C-terminal extracellular region. The protein encoded by this gene forms a homo-oligomeric channel, displays marked permeability to calcium ions and is a major component of brain nicotinic receptors that are blocked by, and highly sensitive to, alpha-bungarotoxin. Once this receptor binds acetylcholine, it undergoes an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. This gene is located in a region identified as a major susceptibility locus for juvenile myoclonic epilepsy and a chromosomal location involved in the genetic transmission of schizophrenia. An evolutionarily recent partial duplication event in this region results in a hybrid containing sequence from this gene and a novel FAM7A gene. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2012]

Uniprot Description

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.