Mouse anti-Human, Rat ASNA1 Monoclonal Antibody | anti-ASNA1 antibody

ASNA1 (ATPase ASNA1, Arsenical Pump-driving ATPase, Arsenite-stimulated ATPase, Transmembrane Domain Recognition Complex 40kD ATPase Subunit, hARSA-I, hASNA-I, ARSA, TRC40) (Biotin)

Cat. Num. AAA6140705

• Gene Names: ASNA1; GET3; ARSA1; TRC40; ARSA-I; ASNA-I
• Reactivity: Human, Rat
• Applications: ELISA, Immunofluorescence, Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ASNA1; Monoclonal Antibody; ASNA1 (ATPase ASNA1; Arsenical Pump-driving ATPase; Arsenite-stimulated ATPase; Transmembrane Domain Recognition Complex 40kD ATPase Subunit; hARSA-I; hASNA-I; ARSA; TRC40) (Biotin); anti-ASNA1 antibody

• Host: Mouse
• Reactivity: Human, Rat
• Clonality: Monoclonal
• Isotype: IgG1,k
• Clone Number: 2H3
• Specificity: Recognizes human ASNA1. Species Crossreactivity: rat.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.

• Applicable Applications for anti-ASNA1 antibody: ELISA (EIA), Immunofluorescence (IF), Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: IF: 10ug/ml; Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa239-348 from human ASNA1 (NP_004308) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: PEQTTFICVCIAEFLSLYETERLIQELAKCKIDTHNIIVNQLVFPDPEKPCKMCEARHKIQAKYLDQMEDLYEDFHIVKLPLLPHEVRGADKVNTFSALLLEPYKPPSAQ
• Conjugate: Biotin
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(ASNA1 monoclonal antibody. Western Blot analysis of ASNA1 expression in PC-12.)

Western Blot (WB)

(Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody. Lane 1: ASNA1 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.5ug/ml])

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged ASNA1 is ~0.03ng/ml as a capture antibody.)

Western Blot (WB)

(Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ASNA1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Western Blot (WB)

(Western Blot detection against Immunogen (37.84kD).)

Product Categories/Family for anti-ASNA1 antibody

Antibodies; Enzymes; ATPase

References

1. A precursor specific role for Hsp40/Hsc70 during tail-anchored protein integration at the endoplasmic reticulum. Rabu C, Wipf P, Brodsky JL, High S.J Biol Chem. 2008 Oct 10;283(41):27504-13. Epub 2008 Jul 29.

NCBI and Uniprot Product Information

• NCBI GI #: 50428938
• NCBI GeneID: 439
• NCBI Accession #: NP_004308
• NCBI GenBank Nucleotide #: NM_004317
• UniProt Accession #: O43681; Q53FC6; Q92849; A6NHP8; A8K740
• Molecular Weight: 38,793 Da
• NCBI Official Full Name: ATPase ASNA1
• NCBI Official Synonym Full Names: arsA arsenite transporter, ATP-binding, homolog 1 (bacterial)
• NCBI Official Symbol: ASNA1
• NCBI Official Synonym Symbols: GET3; ARSA1; TRC40; ARSA-I; ASNA-I
• NCBI Protein Information: ATPase ASNA1
• UniProt Protein Name: ATPase ASNA1
• Protein Family: ATPase
• UniProt Gene Name: ASNA1
• UniProt Entry Name: ASNA_HUMAN

NCBI Description

This gene represents the human homolog of the bacterial arsA gene, encoding the arsenite-stimulated ATPase component of the arsenite transporter responsible for resistance to arsenicals. This protein is also a central component of a transmembrane domain (TMD) recognition complex (TRC) that is involved in the post-translational delivery of tail-anchored (TA) proteins from the cytosol to the endoplasmic reticulum (ER). It recognizes and selectively binds the TMD of TA proteins in the cytosol, and delivers them to the ER for insertion. [provided by RefSeq, Oct 2011]

Uniprot Description

ASNA1: ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting. May be involved in insulin signaling. Belongs to the arsA ATPase family.

Protein type: Nucleolus; EC 3.6.-.-; EC 3.6.3.16; Hydrolase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: endoplasmic reticulum; cytoplasm; nucleolus; nucleus

Molecular Function: arsenite transmembrane transporter activity; transporter activity; metal ion binding; ATPase activity; ATP binding

Biological Process: protein insertion into ER membrane; transport; metabolic process; inorganic anion transport