Human Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) ELISA Kit | CHRNa5 elisa kit

Human Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) ELISA Kit

Cat. Num. AAA2023129

• Gene Names: CHRNA5; LNCR2
• Reactivity: Human
• Synonyms: Cholinergic Receptor; Nicotinic; Alpha 5 (CHRNa5); Human Cholinergic Receptor; Alpha 5 (CHRNa5) ELISA Kit; CHR-NA5; CHRN-A5; N-AChRA5; NAChRA5; N-AChR-A5; Neuronal Acetylcholine Receptor Alpha 5; CHRNa5 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5).; No significant cross-reactivity or interference between Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) and analogues was observed.
• Sequence Length: 468

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.781-50ng/mL
• Sensitivity: < 0.31ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHRNa5 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cholinergic Receptor, Nicotinic, Alpha 5 (CHRNa5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CHRNa5 elisa kit

Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 21707199
• NCBI GeneID: 1138
• UniProt Accession #: P30532; Q15824; Q99554
• Molecular Weight: 53,054 Da
• NCBI Official Full Name: Cholinergic receptor, nicotinic, alpha 5
• NCBI Official Synonym Full Names: cholinergic receptor, nicotinic, alpha 5 (neuronal)
• NCBI Official Symbol: CHRNA5
• NCBI Official Synonym Symbols: LNCR2
• NCBI Protein Information: neuronal acetylcholine receptor subunit alpha-5
• UniProt Protein Name: Neuronal acetylcholine receptor subunit alpha-5
• Protein Family: Neuronal acetylcholine receptor
• UniProt Gene Name: CHRNA5
• UniProt Synonym Gene Names: NACHRA5
• UniProt Entry Name: ACHA5_HUMAN

NCBI Description

The protein encoded by this gene is a nicotinic acetylcholine receptor subunit and a member of a superfamily of ligand-gated ion channels that mediate fast signal transmission at synapses. These receptors are thought to be heteropentamers composed of separate but similar subunits. Defects in this gene have been linked to susceptibility to lung cancer type 2 (LNCR2).[provided by RefSeq, Jun 2010]

Uniprot Description

nAChRA5: After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha- 5/CHRNA5 sub-subfamily.

Protein type: Membrane protein, integral; Channel, ligand-gated; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q24

Cellular Component: nicotinic acetylcholine-gated receptor-channel complex; postsynaptic membrane; plasma membrane; cell junction

Molecular Function: acetylcholine receptor activity; nicotinic acetylcholine-activated cation-selective channel activity; ligand-gated ion channel activity

Biological Process: synaptic transmission; transport; behavioral response to nicotine; signal transduction; cation transport

Disease: Smoking As A Quantitative Trait Locus 3