Guinea Pig PLSCR1 ELISA Kit | PLSCR1 elisa kit
Guinea pig PLSCR1 (Phospholipid Scramblase 1) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to PLSCR1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for PLSCR1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain PLSCR1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of PLSCR1. You can calculate the concentration of PLSCR1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
Uniprot Description
PLSCR1: May mediate accelerated ATP-independent bidirectional transbilayer migration of phospholipids upon binding calcium ions that results in a loss of phospholipid asymmetry in the plasma membrane. May play a central role in the initiation of fibrin clot formation, in the activation of mast cells and in the recognition of apoptotic and injured cells by the reticuloendothelial system. Belongs to the phospholipid scramblase family.
Protein type: Calcium-binding; Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q23
Cellular Component: Golgi apparatus; extracellular matrix; membrane; integral to plasma membrane; plasma membrane; nucleolus; nucleus; cytosol; lipid raft
Molecular Function: phospholipid scramblase activity; protein binding; enzyme binding; DNA binding; calcium ion binding; CD4 receptor binding; SH3 domain binding; epidermal growth factor receptor binding
Biological Process: platelet activation; negative regulation of viral genome replication; apoptosis; positive regulation of innate immune response; acute-phase response; positive regulation of transcription from RNA polymerase II promoter; phospholipid scrambling; defense response to virus; regulation of mast cell activation; phosphatidylserine biosynthetic process
