Human Chemokine C-C-Motif Receptor 7 (CCR7) ELISA Kit | CCR7 elisa kit

Human Chemokine C-C-Motif Receptor 7 (CCR7) ELISA Kit

Cat. Num. AAA2703531

• Gene Names: CCR7; BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
• Reactivity: Human
• Synonyms: Chemokine C-C-Motif Receptor 7 (CCR7); Human Chemokine C-C-Motif Receptor 7 (CCR7) ELISA Kit; CD197; CCR7; BLR2; CDw197; CMKBR7; EBI1; Epstein-Barr Virus Induced Protein 1; EBV-induced G-protein coupled receptor 1; MIP-3 beta receptor; CCR7 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Chemokine C-C-Motif Receptor 7 (CCR7).; No significant cross-reactivity or interference between Chemokine C-C-Motif Receptor 7 (CCR7) and analogues was observed.
• Sequence Length: 378

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 7 (CCR7) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Chemokine C-C-Motif Receptor 7 (CCR7) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCR7 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Chemokine C-C-Motif Receptor 7 (CCR7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Chemokine C-C-Motif Receptor 7 (CCR7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Chemokine C-C-Motif Receptor 7 (CCR7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Chemokine C-C-Motif Receptor 7 (CCR7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4502641
• NCBI GeneID: 1236
• NCBI Accession #: NP_001829.1
• NCBI GenBank Nucleotide #: NM_001838.3
• UniProt Accession #: P32248
• Molecular Weight: 42,874 Da
• NCBI Official Full Name: C-C chemokine receptor type 7 isoform a
• NCBI Official Synonym Full Names: C-C motif chemokine receptor 7
• NCBI Official Symbol: CCR7
• NCBI Official Synonym Symbols: BLR2; EBI1; CCR-7; CD197; CDw197; CMKBR7; CC-CKR-7
• NCBI Protein Information: C-C chemokine receptor type 7
• UniProt Protein Name: C-C chemokine receptor type 7
• Protein Family: C-C chemokine receptor
• UniProt Gene Name: CCR7
• UniProt Synonym Gene Names: CMKBR7; EBI1; EVI1; C-C CKR-7; CC-CKR-7; CCR-7; EBI1; EBV-induced G-protein coupled receptor 1

NCBI Description

The protein encoded by this gene is a member of the G protein-coupled receptor family. This receptor was identified as a gene induced by the Epstein-Barr virus (EBV), and is thought to be a mediator of EBV effects on B lymphocytes. This receptor is expressed in various lymphoid tissues and activates B and T lymphocytes. It has been shown to control the migration of memory T cells to inflamed tissues, as well as stimulate dendritic cell maturation. The chemokine (C-C motif) ligand 19 (CCL19/ECL) has been reported to be a specific ligand of this receptor. Signals mediated by this receptor regulate T cell homeostasis in lymph nodes, and may also function in the activation and polarization of T cells, and in chronic inflammation pathogenesis. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Sep 2014]

Uniprot Description

CCR7: Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions. Belongs to the G-protein coupled receptor 1 family.

Protein type: GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Motility/polarity/chemotaxis; Receptor, GPCR

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cell surface; external side of plasma membrane; integral component of membrane; intracellular; plasma membrane

Molecular Function: C-C chemokine receptor activity; C-C motif chemokine 19 receptor activity; C-C motif chemokine 21 receptor activity; G-protein coupled receptor activity

Biological Process: cellular response to cytokine stimulus; chemokine (C-C motif) ligand 19 signaling pathway; chemokine (C-C motif) ligand 21 signaling pathway; dendritic cell chemotaxis; elevation of cytosolic calcium ion concentration; establishment of T cell polarity; G-protein coupled receptor signaling pathway; homeostasis of number of cells; immune response; inflammatory response; interleukin-12 secretion; myeloid dendritic cell chemotaxis; negative regulation of leukocyte apoptotic process; negative thymic T cell selection; positive regulation of actin filament polymerization; positive regulation of cell adhesion; positive regulation of cell motility; positive regulation of cell-matrix adhesion; positive regulation of dendritic cell antigen processing and presentation; positive regulation of dendritic cell chemotaxis; positive regulation of ERK1 and ERK2 cascade; positive regulation of filopodium formation; positive regulation of glycoprotein biosynthetic process involved in immunological synapse formation; positive regulation of humoral immune response; positive regulation of hypersensitivity; positive regulation of I-kappaB kinase/NF-kappaB signaling; positive regulation of immunological synapse formation; positive regulation of interleukin-12 production; positive regulation of JNK cascade; positive regulation of neutrophil chemotaxis; positive regulation of phosphatidylinositol 3-kinase activity; positive regulation of protein kinase activity; positive regulation of protein kinase B signaling; positive regulation of pseudopodium assembly; positive regulation of T cell costimulation; positive regulation of T cell receptor signaling pathway; regulation of dendritic cell dendrite assembly; regulation of interferon-gamma production; regulation of interleukin-1 beta secretion; release of sequestered calcium ion into cytosol; response to lipopolysaccharide; response to nitric oxide; response to prostaglandin E; ruffle organization