Immunohistochemistry (IHC)
(Immunochemical staining of human PHF17 in human hepatoma with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Rabbit anti-Human JADE1/PHF17 Polyclonal Antibody | anti-JADE1 antibody
Anti-JADE1/PHF17 Antibody, Rabbit Polyclonal
Gene Names
JADE1; PHF17
Reactivity
Human
Applications
Immunohistochemistry, Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
JADE1/PHF17; Polyclonal Antibody; Anti-JADE1/PHF17 Antibody; Rabbit Polyclonal; Jade1/PHF17 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-KIAA1807 Antibody; Anti-PHF17 Antibody; anti-JADE1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human Jade1/PHF17
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-JADE1 antibody
Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
IHC-P: 1:250-1:1000
ICC/IF: 1:300-1:10,000
ICC/IF: 1:300-1:10,000
Immunogen
A synthetic peptide corresponding to the C-terminus of the Human Jade1/PHF17
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the Human Jade1/PHF17, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunohistochemistry (IHC)
(Immunochemical staining of human PHF17 in human hepatoma with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human PHF17 in human hepatoma with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human PHF17 in human liver with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Immunohistochemistry (IHC)
(Immunochemical staining of human PHF17 in human liver with rabbit polyclonal antibody (1:500, formalin-fixed paraffin embedded sections).)
Immunofluorescence (IF)
(Immunofluorescence staining of PHF17 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human PHF17 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of PHF17 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human PHF17 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green).Positive staining was localized to cytoplasm and nucleus.)
Related Product Information for anti-JADE1 antibody
The family of JADE proteins includes three paralogues encoded by individual genes and designated PHF17 (JADE1), PHF16 (JADE2), and PHF15 (JADE3). All three JADE proteins bear in tandem two Plant Homeo-domains (PHDs) which are zinc finger domains. Two isoforms of JADE1, JADE1S, and JADE1L, bind HBO1 and promote acetylation of histones in a chromatin context. The N-terminal region of JADE1 functions as a platform that brings together the catalytic HBO1 subunit with its cognate H3-H4 substrate for histone acetylation. Renal cancer is the major cause of mortality due to abnormal functioning of von Hippel-Lindau (pVHL) and Jade Family PHD Finger 1 (JADE1) complex. E3 ubiquitin ligase JADE1 is stabilized by pVHL interaction through its plant homeodomains (PHDs). JADE1 acts as a renal tumor suppressor that promotes the ubiquitination and degradation of beta-catenin by inhibiting canonical WNT signaling.
References
Siriwardana NS, et al. (2015) The novel function of jade1s in cytokinesis of epithelial cells. Cell Cycle 14 (17): 2821-2834.Shafique S, et al. (2019) Structural basis for renal cancer by the dynamics of pvhl-dependent jade1 stabilization and beta-catenin regulation. Prog Biophys Mol Biol 145 65-77.Panchenko MV (2016) Structure, function and regulation of jade family phd finger 1 (jade1). Gene 589 (1): 1-11.Han J, et al. (2018) The scaffolding protein jade1 physically links the acetyltransferase subunit hbo1 with its histone h3-h4 substrate. J Biol Chem 293 (12): 4498-4509.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
58,370 Da
NCBI Official Full Name
protein Jade-1 isoform 2
NCBI Official Synonym Full Names
jade family PHD finger 1
NCBI Official Symbol
JADE1
NCBI Official Synonym Symbols
PHF17
NCBI Protein Information
protein Jade-1; PHD finger protein 17; PHD protein Jade-1; gene for apoptosis and differentiation in epithelia; jade family PHD finger protein 1
UniProt Protein Name
Protein Jade-1
Protein Family
UniProt Gene Name
JADE1
UniProt Synonym Gene Names
KIAA1807; PHF17
UniProt Entry Name
JADE1_HUMAN
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Product Notes
The JADE1 jade1 (Catalog #AAA8101333) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-JADE1/PHF17 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's JADE1/PHF17 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P), Immunocytochemistry (ICC), Immunofluorescence (IF). IHC-P: 1:250-1:1000 ICC/IF: 1:300-1:10,000. Researchers should empirically determine the suitability of the JADE1 jade1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JADE1/PHF17, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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