Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cell cycle control protein 50A (TMEM30A) Standard Curve.)
Human Cell Cycle Control Protein 50A (TMEM30A) ELISA Kit | TMEM30A elisa kit
Human Cell Cycle Control Protein 50A (TMEM30A) ELISA Kit
Gene Names
TMEM30A; CDC50A; C6orf67
Reactivity
Human
Synonyms
Cell Cycle Control Protein 50A (TMEM30A); Human Cell Cycle Control Protein 50A (TMEM30A) ELISA Kit; TMEM30A; C6orf67; CDC50A; FLJ10856; TMEM30A elisa kit
Reactivity
Human
Specificity
Human Cell cycle control protein 50A (TMEM30A) ELISA Kit has high sensitivity and excellent specificity for detection of Human TMEM30A. No significant cross-reactivity or interference between Human TMEM30A and analogues was observed.
Sequence Length
325
Assay Type
Sandwich ELISA (Quantitative)
Samples
Cell Culture Supernatants, Serum, Plasma, Other Biological Fluids
Detection Method
Colorimetric
Usage Notes
* Do not mix components from different kit lots or use reagents beyond the kit expiration date.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
* Allow all reagents to warm to room temperature for at least 30 minutes before opening.
* Pre-rinse the pipet tip with reagent, use fresh pipet tips for each sample, standard and reagent to avoid contamination.
* Unused wells must be kept desiccated at 4 degree C in the sealed bag provided.
* Mix Thoroughly is very important for the result. It is recommended using low frequency oscillator or slight hand shaking every 10 minutes.
* It is recommended that all samples and standards be assayed in duplicate or triplicate.
Preparation and Storage
Store at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cell cycle control protein 50A (TMEM30A) Standard Curve.)
Typical Testing Data/Standard Curve (for reference only)
(Fig.1. Human Cell cycle control protein 50A (TMEM30A) Standard Curve.)
Related Product Information for TMEM30A elisa kit
Description: This Human Cell cycle control protein 50A (TMEM30A) ELISA Kit employs a two-site sandwich ELISA to quantitate TMEM30A in samples. An antibody specific for TMEM30A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any TMEM30A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for TMEM30A is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of TMEM30A bound in the initial step. The color development is stopped and the intensity of the color is measured.
Background: Antitumor alkylphospholipids initiate apoptosis in transformed HL-60 and Jurkat cells while sparing their progenitors. 1-O-Alkyl-2-carboxymethyl-sn-glycero-3-phosphocholine (Edelfosine) like other short-chained phospholipids--inflammatory platelet-activating factor (PAF) and apoptotic oxidatively truncated phospholipids--are proposed to have intracellular sites of action, yet a conduit for these choline phospholipids into mammalian cells is undefined. Edelfosine is also accumulated by Saccharomyces cerevisiae in a process requiring the membrane protein Lem3p, and the human genome contains a Lem3p homolog TMEM30a. Import of choline phospholipids into S. cerevisiae deltaLem3 is partially reconstituted by human TMEM30a and by Lem3p-TMEM30a chimeras, showing the proteins are orthologous.
Background: Antitumor alkylphospholipids initiate apoptosis in transformed HL-60 and Jurkat cells while sparing their progenitors. 1-O-Alkyl-2-carboxymethyl-sn-glycero-3-phosphocholine (Edelfosine) like other short-chained phospholipids--inflammatory platelet-activating factor (PAF) and apoptotic oxidatively truncated phospholipids--are proposed to have intracellular sites of action, yet a conduit for these choline phospholipids into mammalian cells is undefined. Edelfosine is also accumulated by Saccharomyces cerevisiae in a process requiring the membrane protein Lem3p, and the human genome contains a Lem3p homolog TMEM30a. Import of choline phospholipids into S. cerevisiae deltaLem3 is partially reconstituted by human TMEM30a and by Lem3p-TMEM30a chimeras, showing the proteins are orthologous.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
cell cycle control protein 50A isoform 2
NCBI Official Synonym Full Names
transmembrane protein 30A
NCBI Official Symbol
TMEM30A
NCBI Official Synonym Symbols
CDC50A; C6orf67
NCBI Protein Information
cell cycle control protein 50A
UniProt Protein Name
Cell cycle control protein 50A
Protein Family
UniProt Gene Name
TMEM30A
UniProt Synonym Gene Names
C6orf67; CDC50A
UniProt Entry Name
CC50A_HUMAN
Research Articles on TMEM30A
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Product Notes
The Human TMEM30A tmem30a (Catalog #AAA9713947) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA9713947 ELISA Kit recognizes Human TMEM30A. It is sometimes possible for the material contained within the vial of "Cell Cycle Control Protein 50A (TMEM30A), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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