Human Cell Adhesion Molecule With Homology To L1CAM (CHL1) ELISA Kit | CHL1 elisa kit

Human Cell Adhesion Molecule With Homology To L1CAM (CHL1) ELISA Kit

Cat. Num. AAA2707221

• Gene Names: CHL1; CALL; L1CAM2
• Reactivity: Human
• Synonyms: Cell Adhesion Molecule With Homology To L1CAM (CHL1); Human Cell Adhesion Molecule With Homology To L1CAM (CHL1) ELISA Kit; CALL; L1CAM2; Neural Cell Adhesion Molecule; Cell Adhesion Molecule L1-Like; CHL1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cell Adhesion Molecule With Homology To L1CAM (CHL1).; No significant cross-reactivity or interference between Cell Adhesion Molecule With Homology To L1CAM (CHL1) and analogues was observed.
• Sequence Length: 1224

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.060ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Cell Adhesion Molecule With Homology To L1CAM (CHL1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Cell Adhesion Molecule With Homology To L1CAM (CHL1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CHL1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cell Adhesion Molecule With Homology To L1CAM (CHL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cell Adhesion Molecule With Homology To L1CAM (CHL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cell Adhesion Molecule With Homology To L1CAM (CHL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cell Adhesion Molecule With Homology To L1CAM (CHL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 119584273
• NCBI GeneID: 10752
• Molecular Weight: 136,698 Da
• NCBI Official Full Name: cell adhesion molecule with homology to L1CAM (close homolog of L1), isoform CRA_b
• NCBI Official Synonym Full Names: cell adhesion molecule L1 like
• NCBI Official Symbol: CHL1
• NCBI Official Synonym Symbols: CALL; L1CAM2
• NCBI Protein Information: neural cell adhesion molecule L1-like protein
• UniProt Protein Name: Neural cell adhesion molecule L1-like protein
• Protein Family: ATP-dependent DNA helicase
• UniProt Gene Name: CHL1
• UniProt Synonym Gene Names: CALL

NCBI Description

The protein encoded by this gene is a member of the L1 gene family of neural cell adhesion molecules. It is a neural recognition molecule that may be involved in signal transduction pathways. The deletion of one copy of this gene may be responsible for mental defects in patients with 3p- syndrome. This protein may also play a role in the growth of certain cancers. Alternate splicing results in both coding and non-coding variants. [provided by RefSeq, Nov 2011]

Uniprot Description

CHL1: Extracellular matrix and cell adhesion protein that plays a role in nervous system development and in synaptic plasticity. Both soluble and membranous forms promote neurite outgrowth of cerebellar and hippocampal neurons and suppress neuronal cell death. Plays a role in neuronal positioning of pyramidal neurons and in regulation of both the number of interneurons and the efficacy of GABAergic synapses. May play a role in regulating cell migration in nerve regeneration and cortical development. Potentiates integrin-dependent cell migration towards extracellular matrix proteins. Recruits ANK3 to the plasma membrane. Belongs to the immunoglobulin superfamily. L1/neurofascin/NgCAM family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell adhesion; Cell development/differentiation; Extracellular matrix; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 3p26.3

Cellular Component: apical part of cell; dendrite; integral component of membrane; plasma membrane; proteinaceous extracellular matrix

Molecular Function: protease binding

Biological Process: adult locomotory behavior; axon guidance; axon regeneration; cell adhesion; cognition; negative regulation of neuron apoptosis; neuron migration; signal transduction