Western Blot (WB)
(ARNT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARNT2 antibody detected the ARNT2 protein (arrow).)
Rabbit anti-Human ARNT2 Polyclonal Antibody | anti-ARNT2 antibody
ARNT2, ID (ARNT2, BHLHE1, KIAA0307, Aryl hydrocarbon receptor nuclear translocator 2, Class E basic helix-loop-helix protein 1) (FITC)
FLISA: 1:1,000
Applications are based on unconjugated antibody.
Western Blot (WB)
(ARNT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARNT2 antibody detected the ARNT2 protein (arrow).)
Western Blot (WB)
(ARNT2 Antibody (Center) western blot analysis in 293 cell line lysates (35ug/lane).This demonstrates the ARNT2 antibody detected the ARNT2 protein (arrow).)
Immunofluorescence (IF)
(Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody (Center). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly. )
Immunofluorescence (IF)
(Fluorescent confocal image of SK-BR-3 cell stained with ARNT2 Antibody (Center). SK-BR-3 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with ARNT2 primary antibody (1:25, 1 h at 37 degree C). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree C).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree C). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).ARNT2 immunoreactivity is localized to nucleus significantly. )
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the basic-helix-loop-helix-Per-Arnt-Sim (bHLH-PAS) superfamily of transcription factors. The encoded protein acts as a partner for several sensor proteins of the bHLH-PAS family, forming heterodimers with the sensor proteins that bind regulatory DNA sequences in genes responsive to developmental and environmental stimuli. Under hypoxic conditions, the encoded protein complexes with hypoxia-inducible factor 1alpha in the nucleus and this complex binds to hypoxia-responsive elements in enhancers and promoters of oxygen-responsive genes. A highly similar protein in mouse forms functional complexes with both aryl hydrocarbon receptors and Single-minded proteins, suggesting addition roles for the encoded protein in the metabolism of xenobiotic compounds and the regulation of neurogenesis, respectively. [provided by RefSeq, Jul 2008]
Uniprot Description
ARNT2: Specifically recognizes the xenobiotic response element (XRE). 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Transcription factor; DNA-binding; Apoptosis
Chromosomal Location of Human Ortholog: 15q24
Cellular Component: transcription factor complex; cytoplasm; nucleus
Molecular Function: signal transducer activity; DNA binding; aryl hydrocarbon receptor binding; protein heterodimerization activity; transcription factor activity
Biological Process: central nervous system development; regulation of transcription, DNA-dependent; transcription, DNA-dependent; in utero embryonic development; positive regulation of transcription, DNA-dependent; positive regulation of cell proliferation; response to hypoxia; positive regulation of transcription from RNA polymerase II promoter; brain development; signal transduction; response to estradiol stimulus; negative regulation of apoptosis
Disease: Webb-dattani Syndrome
Research Articles on ARNT2
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Product Notes
The ARNT2 arnt2 (Catalog #AAA6275328) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ARNT2, ID (ARNT2, BHLHE1, KIAA0307, Aryl hydrocarbon receptor nuclear translocator 2, Class E basic helix-loop-helix protein 1) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ARNT2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), FLISA. WB: 1:100-500 FLISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ARNT2 arnt2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ARNT2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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