Human CD93/C1qR ELISA Kit | CD93 elisa kit

Human CD93/C1qR PicoKine ELISA Kit

Cat. Num. AAA177175

• Gene Names: CD93; C1QR1; C1qRP; CDw93; ECSM3; MXRA4; C1qR(P); dJ737E23.1
• Reactivity: Human
• Synonyms: CD93/C1qR; Human CD93/C1qR PicoKine ELISA Kit; Complement component C1q receptor; CD93 molecule; C1q receptor 1; C1q/MBL/SPA receptor; C1qR; C1qR(p); C1qr1; C1QR1_HUMAN; C1qRp; CD93; CD93 antigen; CDw93; Complement component 1 q subcomponent receptor 1; dJ737E23.1; ECSM3; Matrix remodeling associated protein 4; Matrix remodelling associated 4; Matrix-remodeling-associated protein 4; MXRA4; CD93 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CD93
• Sequence Length: 652

• Samples: Cell culture supernates, serum, plasma (heparin, EDTA), saliva and urine
• Assay Type: Sandwich
• Detection Range: 78pg/ml-5000pg/ml
• Sensitivity: < 10pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human CD93/C1qR PicoKine ELISA Kit standard curve )

Related Product Information for CD93 elisa kit

Principle of the assay: human CD93 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CD93 has been precoated onto 96-well plates. Standards(NSO, A24-K580) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CD93 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CD93 amount of sample captured in plate.
Background: CD93 (Cluster of Differentiation 93) is a protein that in humans is encoded by the CD93 gene. CD93 belongs to the Group XIV C-Type lectin family and is mapped to 20p11.21. The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. It has also showed that this receptor is a positive marker for all bone marrow-repopulating stem cells because it is expressed on both CD34-negative and CD34-positive stem cells from umbilical cord blood and adult bone marrow. In addition, highly purified lineage-negative cells with the CD93 protein not only have bone marrow-repopulating capacity but also can differentiate into human hepatocytes in vivo.

NCBI and Uniprot Product Information

• NCBI GI #: 21759074
• NCBI GeneID: 22918
• UniProt Accession #: Q9NPY3; O00274
• Molecular Weight: 68,560 Da
• NCBI Official Full Name: Complement component C1q receptor
• NCBI Official Synonym Full Names: CD93 molecule
• NCBI Official Symbol: CD93
• NCBI Official Synonym Symbols: C1QR1; C1qRP; CDw93; ECSM3; MXRA4; C1qR(P); dJ737E23.1
• NCBI Protein Information: complement component C1q receptor; C1qR; CD93 antigen; C1q receptor 1; C1q/MBL/SPA receptor; matrix-remodelling associated 4; matrix-remodeling-associated protein 4; complement component 1 q subcomponent receptor 1; complement component 1, q subcomponent, receptor 1
• UniProt Protein Name: Complement component C1q receptor
• Protein Family: Complement component C1q receptor
• UniProt Gene Name: CD93
• UniProt Synonym Gene Names: C1QR1; MXRA4; C1qR; C1qR(p); C1qRp
• UniProt Entry Name: C1QR1_HUMAN

NCBI Description

The protein encoded by this gene is a cell-surface glycoprotein and type I membrane protein that was originally identified as a myeloid cell-specific marker. The encoded protein was once thought to be a receptor for C1q, but now is thought to instead be involved in intercellular adhesion and in the clearance of apoptotic cells. The intracellular cytoplasmic tail of this protein has been found to interact with moesin, a protein known to play a role in linking transmembrane proteins to the cytoskeleton and in the remodelling of the cytoskeleton. [provided by RefSeq, Jul 2008]

Uniprot Description

CD93: Receptor (or element of a larger receptor complex) for C1q, mannose-binding lectin (MBL2) and pulmonary surfactant protein A (SPA). May mediate the enhancement of phagocytosis in monocytes and macrophages upon interaction with soluble defense collagens. May play a role in intercellular adhesion.

Protein type: Membrane protein, integral; Cell adhesion

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: cell surface; cytoplasmic membrane-bound vesicle; plasma membrane; integral to membrane

Molecular Function: protein binding; complement component C1q binding; receptor activity; calcium ion binding; carbohydrate binding

Biological Process: macrophage activation; cell-cell adhesion; viral reproduction; phagocytosis