Human CCL13/MCP4 ELISA Kit | CCL13 elisa kit

Human CCL13/MCP4 PicoKine ELISA Kit

Cat. Num. AAA177198

• Gene Names: CCL13; NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
• Reactivity: Human
• Synonyms: CCL13/MCP4; Human CCL13/MCP4 PicoKine ELISA Kit; C-C motif chemokine 13; chemokine (C-C motif) ligand 13; CCL13; CCL13_HUMAN; Chemokine (C C motif) ligand 13; CK beta 10; CK-beta-10; CKb10; Macrophage/monocyte chemotactic protein 4; MCP-4; MGC17134; Monocyte chemoattractant protein 4; Monocyte chemotactic protein 4; NCC 1; NCC-1; NCC1; New CC chemokine 1; SCYA13; SCYL1; short chain; Small inducible cytokine A13; Small inducible cytokine A13 precursor; Small inducible cytokine subfamily A (Cys Cys) member 13; Small-inducible cytokine A13; CCL13 elisa kit

• Reactivity: Human
• Specificity: Natural and recombinant human CCL13
• Sequence Length: 98

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 7.8pg/ml-500pg/ml
• Sensitivity: < 10 pg/ml
• Intra-assay Precision: Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
• Inter-assay Precision: Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Human CCL13/MCP4 PicoKine ELISA Kit standard curve )

Related Product Information for CCL13 elisa kit

Principle of the assay: human CCL13 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for CCL13 has been precoated onto 96-well plates. Standards(E.coli, Q24-T98) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for CCL13 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human CCL13 amount of sample captured in plate.
Background: Chemokine (C-C motif) ligand 13 (CCL13) is a small cytokine belonging to the CC chemokine family. Its gene is located on human chromosome 17 within a large cluster of other CC chemokines. CCL13 induces chemotaxis in monocytes, eosinophils, T lymphocytes, and basophils by binding cell surface G-protein linked chemokine receptors such as CCR2, CCR3 and CCR5. Activity of this chemokine has been implicated in allergic reactions such as asthma. CCL13 can be induced by the inflammatory cytokines interleukin-1 and TNF-alpha.

NCBI and Uniprot Product Information

• NCBI GI #: 3024128
• NCBI GeneID: 6357
• UniProt Accession #: Q99616; O95689; Q6ICQ6
• Molecular Weight: 10,986 Da
• NCBI Official Full Name: C-C motif chemokine 13
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 13
• NCBI Official Symbol: CCL13
• NCBI Official Synonym Symbols: NCC1; CKb10; MCP-4; NCC-1; SCYL1; SCYA13
• NCBI Protein Information: C-C motif chemokine 13; CK-beta-10; new CC chemokine 1; small-inducible cytokine A13; monocyte chemotactic protein 4; monocyte chemoattractant protein 4; small inducible cytokine subfamily A (Cys-Cys), member 13
• UniProt Protein Name: C-C motif chemokine 13
• Protein Family: C-C motif chemokine
• UniProt Gene Name: CCL13
• UniProt Synonym Gene Names: MCP4; NCC1; SCYA13; MCP-4
• UniProt Entry Name: CCL13_HUMAN

NCBI Description

This antimicrobial gene is one of several Cys-Cys (CC) cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. The cytokine encoded by this gene displays chemotactic activity for monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. This chemokine plays a role in accumulation of leukocytes during inflammation. It may also be involved in the recruitment of monocytes into the arterial wall during artherosclerosis. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL13: Chemotactic factor that attracts monocytes, lymphocytes, basophils and eosinophils, but not neutrophils. Signals through CCR2B and CCR3 receptors. Plays a role in the accumulation of leukocytes at both sides of allergic and non-allergic inflammation. May be involved in the recruitment of monocytes into the arterial wall during the disease process of atherosclerosis. May play a role in the monocyte attraction in tissues chronically exposed to exogenous pathogens. By IL1/interleukin-1 and TNF. Widely expressed. Found in small intestine, thymus, colon, lung, trachea, stomach and lymph node. Low levels seen in the pulmonary artery smooth muscle cells. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Chemokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 17q11.2

Cellular Component: extracellular space

Molecular Function: chemokine activity; receptor binding

Biological Process: cellular calcium ion homeostasis; regulation of cell shape; cell-cell signaling; eosinophil chemotaxis; immune response; cytoskeleton organization and biogenesis; chemotaxis; inflammatory response; signal transduction