Human Carbonic Anhydrase XII (CA12) ELISA Kit | CA12 elisa kit

Human Carbonic Anhydrase XII (CA12) ELISA Kit

Cat. Num. AAA2022841

• Gene Names: CA12; CAXII; HsT18816
• Reactivity: Human
• Synonyms: Carbonic Anhydrase XII (CA12); Human Carbonic Anhydrase XII (CA12) ELISA Kit; CA-XII; CAXII; Carbonate dehydratase XII; Tumor antigen HOM-RCC-3.1.3; CA12 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase XII (CA12).; No significant cross-reactivity or interference between Carbonic Anhydrase XII (CA12) and analogues was observed.
• Sequence Length: 343

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernates and Other Biological Fluids
• Detection Range: 15.6-1000pg/mL
• Sensitivity: < 6.8pg/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Carbonic Anhydrase XII (CA12) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Carbonic Anhydrase XII (CA12) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CA12 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase XII (CA12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase XII (CA12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase XII (CA12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase XII (CA12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for CA12 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 2984693
• NCBI GeneID: 771
• UniProt Accession #: O43570; Q53YE5; Q9BWG2; B2RE24
• Molecular Weight: 38,408 Da
• NCBI Official Full Name: carbonic anhydrase XII
• NCBI Official Synonym Full Names: carbonic anhydrase XII
• NCBI Official Symbol: CA12
• NCBI Official Synonym Symbols: CAXII; HsT18816
• NCBI Protein Information: carbonic anhydrase 12
• UniProt Protein Name: Carbonic anhydrase 12
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA12
• UniProt Synonym Gene Names: CA-XII
• UniProt Entry Name: CAH12_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Three transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq, Jun 2014]

Uniprot Description

CA12: Reversible hydration of carbon dioxide. Homodimer. Highly expressed in colon, kidney, prostate, intestine and activated lymphocytes. Expressed at much higher levels in the renal cell cancers than in surrounding normal kidney tissue. Moderately expressed in pancreas, ovary and testis. Inhibited by coumarins, saccharin, sulfonamide derivatives such as acetazolamide (AZA), benzenesulfonamide and derivatives (4-carboxyethylbenzene-sulfonamide, 4- carboxyethylbenzene-sulfonamide ethyl ester, 4-(acetyl-2- aminoethyl)benzene-sulfonamide, 4-aminoethylbenzene-sulfonamide) and Foscarnet (phosphonoformate trisodium salt). Belongs to the alpha-carbonic anhydrase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.2.1.1; Membrane protein, integral; Energy Metabolism - nitrogen; Lyase

Chromosomal Location of Human Ortholog: 15q22

Cellular Component: integral to membrane; plasma membrane

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process; chloride ion homeostasis

Disease: Hyperchlorhidrosis, Isolated