Typical Testing Data/Standard Curve (for reference only)
Human Cannabinoid receptor 2 (CNR2) ELISA Kit | CNR2 elisa kit
Human Cannabinoid receptor 2 (CNR2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: CNR2 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CNR2 antibody and an CNR2-HRP conjugate. The assay sample and buffer are incubated together with CNR2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNR2 concentration since CNR2 from samples and CNR2-HRP conjugate compete for the anti-CNR2 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNR2 from the sample, fewer sites are left to bind CNR2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNR2 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The cannabinoid delta-9-tetrahydrocannabinol is the principal psychoactive ingredient of marijuana. The proteins encoded by this gene and the cannabinoid receptor 1 (brain) (CNR1) gene have the characteristics of a guanine nucleotide-binding protein (G-protein)-coupled receptor for cannabinoids. They inhibit adenylate cyclase activity in a dose-dependent, stereoselective, and pertussis toxin-sensitive manner. These proteins have been found to be involved in the cannabinoid-induced CNS effects (including alterations in mood and cognition) experienced by users of marijuana. The cannabinoid receptors are members of family 1 of the G-protein-coupled receptors. [provided by RefSeq, Jul 2008]
Uniprot Description
CNR2: Heterotrimeric G protein-coupled receptor for endocannabinoid 2-arachidonoylglycerol mediating inhibition of adenylate cyclase. May function in inflammatory response, nociceptive transmission and bone homeostasis. Belongs to the G-protein coupled receptor 1 family.
Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass
Chromosomal Location of Human Ortholog: 1p36.11
Cellular Component: extrinsic to internal side of plasma membrane; integral to plasma membrane; dendrite; plasma membrane; perikaryon
Molecular Function: cannabinoid receptor activity
Biological Process: negative regulation of mast cell activation; G-protein signaling, coupled to cyclic nucleotide second messenger; negative regulation of nitric-oxide synthase activity; negative regulation of inflammatory response; response to amphetamine; response to lipopolysaccharide; immune response; negative regulation of action potential; sensory perception of pain; inflammatory response; negative regulation of synaptic transmission, GABAergic
Research Articles on CNR2
Similar Products
Product Notes
The Human CNR2 cnr2 (Catalog #AAA7244347) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7244347 ELISA Kit recognizes Human CNR2. It is sometimes possible for the material contained within the vial of "Cannabinoid receptor 2 (CNR2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
