Human Calponin 3 (CNN3) ELISA Kit | CNN3 elisa kit

Human Calponin 3 (CNN3) ELISA Kit

Cat. Num. AAA7210862

• Reactivity: Human
• Synonyms: Calponin 3 (CNN3); Human Calponin 3 (CNN3) ELISA Kit; CNN3 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of CNN3. No significant cross-reactivity or interference between CNN3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CNN3 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CNN3 elisa kit

Intended Uses: This CNN3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCNN3. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: CNN3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CNN3 antibody and an CNN3-HRP conjugate. The assay sample and buffer are incubated together with CNN3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CNN3 concentration since CNN3 from samples and CNN3-HRP conjugate compete for the anti-CNN3 antibody binding site. Since the number of sites is limited, as more sites are occupied by CNN3 from the sample, fewer sites are left to bind CNN3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CNN3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CNN3 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 554790263
• NCBI GeneID: 1266
• NCBI Accession #: NP_001272985.1
• NCBI GenBank Nucleotide #: NM_001286056.1
• Molecular Weight: 31,380 Da
• NCBI Official Full Name: calponin-3 isoform 3
• NCBI Official Synonym Full Names: calponin 3, acidic
• NCBI Official Symbol: CNN3
• NCBI Protein Information: calponin-3; calponin, acidic isoform; dJ639P13.2.2 (acidic calponin 3)
• UniProt Protein Name: Calponin-3
• Protein Family: Calponin
• UniProt Gene Name: CNN3
• UniProt Entry Name: CNN3_HUMAN

NCBI Description

This gene encodes a protein with a markedly acidic C terminus; the basic N-terminus is highly homologous to the N-terminus of a related gene, CNN1. Members of the CNN gene family all contain similar tandemly repeated motifs. This encoded protein is associated with the cytoskeleton but is not involved in contraction. [provided by RefSeq, Jul 2008]

Uniprot Description

calponin 3: Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin, troponin C and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity. Belongs to the calponin family.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 1p22-p21

Cellular Component: focal adhesion; cytoplasm; actin cytoskeleton

Molecular Function: calmodulin binding; actin binding

Biological Process: epithelial cell differentiation; actomyosin structure organization and biogenesis