Typical Testing Data/Standard Curve (for reference only)
Human Cadherin related family member 5 (MUPCDH) ELISA Kit | MUPCDH elisa kit
Human Cadherin related family member 5 (MUPCDH) ELISA Kit
Gene Names
CDHR5; MUCDHL; MUPCDH; MU-PCDH
Reactivity
Human
Synonyms
Cadherin related family member 5 (MUPCDH); Human Cadherin related family member 5 (MUPCDH) ELISA Kit; MUPCDH elisa kit
Reactivity
Human
Specificity
This assay has high sensitivity and excellent specificity for detection of CDHR5. No significant cross-reactivity or interference between CDHR5 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CDHR5 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for MUPCDH elisa kit
Intended Uses: This CDHR5 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of HumanCDHR5. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: CDHR5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDHR5 antibody and an CDHR5-HRP conjugate. The assay sample and buffer are incubated together with CDHR5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDHR5 concentration since CDHR5 from samples and CDHR5-HRP conjugate compete for the anti-CDHR5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDHR5 from the sample, fewer sites are left to bind CDHR5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDHR5 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CDHR5 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CDHR5 antibody and an CDHR5-HRP conjugate. The assay sample and buffer are incubated together with CDHR5-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CDHR5 concentration since CDHR5 from samples and CDHR5-HRP conjugate compete for the anti-CDHR5 antibody binding site. Since the number of sites is limited, as more sites are occupied by CDHR5 from the sample, fewer sites are left to bind CDHR5-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CDHR5 concentration in each sample is interpolated from this standard curve.
Product Categories/Family for MUPCDH elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
87,631 Da
NCBI Official Full Name
Cadherin-related family member 5
NCBI Official Synonym Full Names
cadherin-related family member 5
NCBI Official Symbol
CDHR5
NCBI Official Synonym Symbols
MUCDHL; MUPCDH; MU-PCDH
NCBI Protein Information
cadherin-related family member 5; mu-protocadherin; mucin and cadherin-like protein
UniProt Protein Name
Cadherin-related family member 5
UniProt Gene Name
CDHR5
UniProt Synonym Gene Names
MUCDHL; MUPCDH; UNQ2781/PRO7168
UniProt Entry Name
CDHR5_HUMAN
NCBI Description
This gene is a novel mucin-like gene that is a member of the cadherin superfamily. While encoding nonpolymorphic tandem repeats rich in proline, serine and threonine similar to mucin proteins, the gene also contains sequence encoding calcium-binding motifs found in all cadherins. The role of the hybrid extracellular region and the specific function of this protein have not yet been determined. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq, Jan 2010]
Uniprot Description
MUCDHL: Acts as a calcium-dependent cell adhesion protein. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, integral; Motility/polarity/chemotaxis; Cell adhesion
Chromosomal Location of Human Ortholog: 11p15.5
Cellular Component: plasma membrane; integral to membrane
Molecular Function: protein binding; beta-catenin binding; calcium ion binding
Biological Process: cell adhesion; homophilic cell adhesion
Research Articles on MUPCDH
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Product Notes
The Human MUPCDH cdhr5 (Catalog #AAA7222204) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7222204 ELISA Kit recognizes Human MUPCDH. It is sometimes possible for the material contained within the vial of "Cadherin related family member 5 (MUPCDH), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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