Rabbit Procollagen Type III ELISA Kit | PC3 elisa kit

Rabbit Procollagen Type III ELISA Kit

• Gene Names: CHMP1A; PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
• Reactivity: Rabbit
• Synonyms: Procollagen Type III; Rabbit Procollagen Type III ELISA Kit; PC3 elisa kit

• Reactivity: Rabbit
• Specificity: No significant cross-reactivity or interference between Rabbit PC3 and analogues was observed.
• Sequence Length: 332

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 6.25 ng/ml - 200 ng/ml.
• Sensitivity: 1.0 ng/ml.
• Intended Uses: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate PC3 concentrations in Rabbit serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research, not for drug, household, therapeutic applications
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean x100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PC3 elisa kit

Introduction: ELISA is a simple and highly sensitive method of analysis that allows for simultaneous and rapid quantification of a large number of samples. The assay is based on the specific recognition of the target compound (analyte/antigen) by antibodies which bind to the compound. The antigen-antibody complex is detected and measured with the aid of an enzyme-labeled antibody or antigen. Upon addition of a non-colored reagent, the enzyme produces a color reaction where the color intensity is directly or inversely proportional to the concentration of the analyte in the sample. This quantitative Sandwich ELISA kit is in tended to determinate PC3 concentrations in Rabbit serum, plasma, tissue homogenates, feces, urine and body fluids, and it is only for research!

NCBI and Uniprot Product Information

• NCBI GI #: 119587121
• NCBI GeneID: 5119
• UniProt Accession #: Q9HD42; Q14468; Q15779; Q96G31; A2RU09
• Molecular Weight: 21,703 Da
• NCBI Official Full Name: procollagen (type III) N-endopeptidase, isoform CRA_a, partial
• NCBI Official Synonym Full Names: charged multivesicular body protein 1A
• NCBI Official Symbol: CHMP1A
• NCBI Official Synonym Symbols: PCH8; CHMP1; PRSM1; PCOLN3; VPS46A; VPS46-1
• NCBI Protein Information: charged multivesicular body protein 1a; protease, metallo, 1, 33kD; chromatin modifying protein 1A; procollagen (type III) N-endopeptidase; vacuolar protein sorting-associated protein 46-1; charged multivesicular body protein 1/chromatin modifying protein 1
• UniProt Protein Name: Charged multivesicular body protein 1a
• UniProt Gene Name: CHMP1A
• UniProt Synonym Gene Names: CHMP1; KIAA0047; PCOLN3; PRSM1; CHMP1a; Vps46-1; hVps46-1
• UniProt Entry Name: CHM1A_HUMAN

NCBI Description

This gene encodes a member of the CHMP/Chmp family of proteins which are involved in multivesicular body sorting of proteins to the interiors of lysosomes. The initial prediction of the protein sequence encoded by this gene suggested that the encoded protein was a metallopeptidase. The nomenclature has been updated recently to reflect the correct biological function of this encoded protein. Several transcripts encoding different isoforms have been found for this gene. [provided by RefSeq, Dec 2012]

Uniprot Description

CHMP1A: Probable peripherally associated component of the endosomal sorting required for transport complex III (ESCRT-III) which is involved in multivesicular bodies (MVBs) formation and sorting of endosomal cargo proteins into MVBs. MVBs contain intraluminal vesicles (ILVs) that are generated by invagination and scission from the limiting membrane of the endosome and mostly are delivered to lysosomes enabling degradation of membrane proteins, such as stimulated growth factor receptors, lysosomal enzymes and lipids. The MVB pathway appears to require the sequential function of ESCRT-O, -I,-II and -III complexes. ESCRT- III proteins mostly dissociate from the invaginating membrane before the ILV is released. The ESCRT machinery also functions in topologically equivalent membrane fission events, such as the terminal stages of cytokinesis and the budding of enveloped viruses (HIV-1 and other lentiviruses). ESCRT-III proteins are believed to mediate the necessary vesicle extrusion and/or membrane fission activities, possibly in conjunction with the AAA ATPase VPS4. Involved in cytokinesis. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. May also be involved in chromosome condensation. Targets the Polycomb group (PcG) protein BMI1/PCGF4 to regions of condensed chromatin. May play a role in stable cell cycle progression and in PcG gene silencing. Belongs to the SNF7 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Cell cycle regulation

Chromosomal Location of Human Ortholog: 16q24.3

Cellular Component: condensed nuclear chromosome; nuclear matrix; early endosome; endomembrane system; microtubule organizing center

Molecular Function: protein domain specific binding; protein binding; protein homodimerization activity; zinc ion binding; metallopeptidase activity

Biological Process: vesicle-mediated transport; protein transport; transcription, DNA-dependent; cell separation during cytokinesis; negative regulation of transcription by glucose; mitotic chromosome condensation; vacuolar transport; cytokinesis; gene silencing; proteolysis; negative regulation of transcription, DNA-dependent; mitotic metaphase plate congression; nuclear organization and biogenesis

Disease: Pontocerebellar Hypoplasia, Type 8