Human CA1 ELISA Kit | CA1 elisa kit

Human CA1 (Carbonic Anhydrase I) ELISA Kit

Cat. Num. AAA2511363

• Gene Names: CA1; CAB; CA-I; Car1; HEL-S-11
• Reactivity: Human
• Synonyms: CA1; Human CA1 (Carbonic Anhydrase I) ELISA Kit; CA1 elisa kit

• Reactivity: Human
• Specificity: This kit recognizes Human C1q in samples. No Significant cross-reactivity or interference between Human C1q and analogues was observed.
• Sequence Length: 261

• Samples: Serum, Plasma And Other Biological Fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 78.13-5000 pg/mL
• Sensitivity: 46.88 pg/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, mid range and high level Human C1q were tested 20 times on one plate, respectively
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, mid range and high level Human C1q were tested on 3 different plates, 20 replicates in each plate.
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CA1 elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Human C1q concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses the Sandwich-ELISA principle. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Human C1q. Standards or samples are added to the micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for Human C1q and Avidin-Horseradish Peroxidase (HRP) conjugate are added successively to each micro plate well and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Human C1q, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of stop solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Human C1q. You can calculate the concentration of Human C1q in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 192447434
• NCBI GeneID: 759
• NCBI Accession #: NP_001122303.1
• NCBI GenBank Nucleotide #: NM_001128831.3
• UniProt Accession #: P00915
• Molecular Weight: 28,870 Da
• NCBI Official Full Name: carbonic anhydrase 1 isoform a
• NCBI Official Synonym Full Names: carbonic anhydrase I
• NCBI Official Symbol: CA1
• NCBI Official Synonym Symbols: CAB; CA-I; Car1; HEL-S-11
• NCBI Protein Information: carbonic anhydrase 1; carbonate dehydratase I; carbonic anhydrase B; epididymis secretory protein Li 11
• UniProt Protein Name: Carbonic anhydrase 1
• Protein Family: Cation-transporting ATPase
• UniProt Gene Name: CA1
• UniProt Synonym Gene Names: CAB; CA-I
• UniProt Entry Name: CAH1_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This CA1 gene is closely linked to the CA2 and CA3 genes on chromosome 8. It encodes a cytosolic protein that is found at the highest level in erythrocytes. Allelic variants of this gene have been described in some populations. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CA1: Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 8q21.2

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; one-carbon compound metabolic process