Rabbit VR1 Polyclonal Antibody | anti-VR1 antibody

VR1 Antibody

Cat. Num. AAA9603178

• Gene Names: TRPV1; VR1
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: VR1; Polyclonal Antibody; VR1 Antibody; Capsaicin receptor; DKFZp434K0220; osm 9 like TRP channel 1; Osm-9-like TRP channel 1; OTRPC1; Transient receptor potential cation channel subfamily V member 1; TRPV 1; Trpv1; TRPV1_HUMAN; Vanilloid receptor 1; Vanilloid receptor subtype 1; VR 1; anti-VR1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: VR1 antibody detects endogenous levels of VR1
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 839

• Applicable Applications for anti-VR1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthesized peptide derived from human VR1
• Subcellular Location: Cell Junction > Synapse > Postsynaptic Cell Membrane. Cell Projection > Dendritic Spine Membrane.
• Tissue Specificity: Widely expressed at low levels. Expression is elevated in dorsal root ganglia. In skin, expressed in cutaneous sensory nerve fibers, mast cells, epidermal keratinocytes, dermal blood vessels, the inner root sheet and the infundibulum of hair follicles, differentiated sebocytes, sweat gland ducts, and the secretory portion of eccrine sweat glands (at protein level).
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts of Human skin  tissue sample, using VR1 Antibody(MBS9603178).)

Related Product Information for anti-VR1 antibody

Function: Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time-and Ca2+-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH < 6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis.
Subunit Structure: Interacts with PIRT (By similarity). Homotetramer (By similarity). Interacts with TRPV3 and may also form a heteromeric channel with TRPV3 (PubMed:12077606). Interacts with CALM, PRKCM and CSK. Interacts with PRKCG and NTRK1, probably by forming a trimeric complex (By similarity). Interacts with the Scolopendra mutilans RhTx toxin (By similarity). Interacts with TMEM100 (By similarity).
Post-translational Modifications: Phosphorylation by PKA reverses capsaicin-induced dephosphorylation at multiple sites, probably including Ser-117 as a major phosphorylation site. Phosphorylation by CAMKII seems to regulate binding to vanilloids. Phosphorylated and modulated by PRKCE, PRKCM and probably PRKCZ. Dephosphorylation by calcineurin seems to lead to receptor desensitization and phosphorylation by CAMKII recovers activity.
Similarity: The association domain (AD) is necessary for self-association. Belongs to the transient receptor (TC 1.A.4) family. TrpV subfamily. TRPV1 sub-subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 74315350
• NCBI GeneID: 7442
• NCBI Accession #: NP_061197.4
• NCBI GenBank Nucleotide #: NM_018727.5
• UniProt Accession #: Q8NER1; Q3LU47; Q9H0G9; Q9H303; Q9H304; Q9NQ74; Q9NY22; A2RUA9
• Molecular Weight: Observed: 95 kDa; Predicted: 95 kDa
• NCBI Official Full Name: transient receptor potential cation channel subfamily V member 1
• NCBI Official Synonym Full Names: transient receptor potential cation channel subfamily V member 1
• NCBI Official Symbol: TRPV1
• NCBI Official Synonym Symbols: VR1
• NCBI Protein Information: transient receptor potential cation channel subfamily V member 1
• UniProt Protein Name: Transient receptor potential cation channel subfamily V member 1
• UniProt Gene Name: TRPV1
• UniProt Synonym Gene Names: VR1; TrpV1; OTRPC1

NCBI Description

Capsaicin, the main pungent ingredient in hot chili peppers, elicits a sensation of burning pain by selectively activating sensory neurons that convey information about noxious stimuli to the central nervous system. The protein encoded by this gene is a receptor for capsaicin and is a non-selective cation channel that is structurally related to members of the TRP family of ion channels. This receptor is also activated by increases in temperature in the noxious range, suggesting that it functions as a transducer of painful thermal stimuli in vivo. Four transcript variants encoding the same protein, but with different 5' UTR sequence, have been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Ligand-activated non-selective calcium permeant cation channel involved in detection of noxious chemical and thermal stimuli. Seems to mediate proton influx and may be involved in intracellular acidosis in nociceptive neurons. Involved in mediation of inflammatory pain and hyperalgesia. Sensitized by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases, which involves PKC isozymes and PCL. Activation by vanilloids, like capsaicin, and temperatures higher than 42 degrees Celsius, exhibits a time- and Ca2+-dependent outward rectification, followed by a long-lasting refractory state. Mild extracellular acidic pH (6.5) potentiates channel activation by noxious heat and vanilloids, whereas acidic conditions (pH <6) directly activate the channel. Can be activated by endogenous compounds, including 12-hydroperoxytetraenoic acid and bradykinin. Acts as ionotropic endocannabinoid receptor with central neuromodulatory effects. Triggers a form of long-term depression (TRPV1-LTD) mediated by the endocannabinoid anandamine in the hippocampus and nucleus accumbens by affecting AMPA receptors endocytosis.